饮酒对大鼠生精细胞iNOS,Bcl-2基因表达的影响

目的 探讨酒精对大鼠睾丸诱导型一氧化氮合酶（iNOS）、Bcl-2基因表达和生精细胞凋亡的影响。方法30只成年健康SD雄性大鼠随机均分为对照组、低剂量组和高剂量组，用不同剂量的酒精灌胃成年大鼠26d（两个生精周期）后，免疫组织化学法（SP法）检测睾丸iNOS、BCl-2基因表达的变化；原位缺口末端标记法（TUNEL法）检测细胞凋亡指数（A1）的变化。结果 与对照组相比，低剂量组大鼠睾丸iNOS、Bcl-2基闪表达强度和细胞凋亡指数（A1）无明显变化（P〉0．05）：而高剂量组与对照组和低剂量组相比，iNOS表达显著增强（P〈0．01），Bcl-2基因表达明显减弱（P〈0．01，P〈0．05），细胞凋亡指数则增加（P〈0.01）。结论 长期大量饮酒可以诱导睾丸生精细胞凋亡增加，iNOS与Bcl-2基因表达的改变是重要原因之一。

Impacts of alcohol intake on expression of iNOS and Bcl-2 gene in spermatogenic cells of rat

Objective To investigate tha alterations of iNOS, Bcl-2 and the spermatogenic cells apoptosis in therat testis submitted to alcohol. Methods Thirty healthy male Sprague-Davwley rats were randomly divided equallyinto three groups: control group, low dose group and high dose group. Alcohol was administered intragastrically atdifferent doses to three groups of adult rats respectively. After twenty-six days, the immunohistochemical stainingmethod (SP method) for detecting the expression of iNOS and Bcl-2 in the testis and TUNEL(TdT-mediateddUTP-X nick end labeling) method for detecting AI (apoptotic index) were used in the present study. ResultsCompared with control group, no significant differences were noted in the expression of iNOS, Bcl-2 and AI in low dosegroup (P>0.05); but the expression of iNOS increased significantly (P<0.01) and that of Bcl-2 decreasedmarkedly( P<0.05, P<0.01), the AI of spermatogenic cells in high dose group was predominantly higher than thatin low dose and control group(P<0.01). Conclusion The expression variations of iNOS and Bcl-2 is one of maincauses that accohol overtaking induces spermatogenic cells apoptosis.