Ethanol modulates GABA(B) receptor expression in cortex and hippocampus of the adult rat brain |
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Authors: | Li Shu Peng Park Moon Seok Jin Guang Zhen Kim Jong Hun Lee Hye Lyoung Lee Yu La Kim Jin Hoi Bahk Jong Yoon Park Tae Ju Koh Phil Ok Chung Bong Chul Kim Myeong Ok |
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Affiliation: | Division of Life Science and Applied Life Science (Brain Korea 21), Gyeongsang National University, Chinju 660-701, South Korea. |
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Abstract: | Using in situ hybridization, RNase protection assay and Western blot, we studied the effects of ethanol on the expression levels of GABA(B) receptor mRNA and protein in the cortex and hippocampus from adult rat brain. The results showed that ethanol significantly increased GABA(B1) and GABA(B2) receptor protein expression in the cortex, whereas only GABA(B2) was increased in the hippocampus. GABA(B) receptor agonist baclofen could partially reverse the effect of ethanol. Further studies of the mRNA levels defined that GABA(B1) mRNA levels were significantly increased in the hippocampus, with no significant changes of GABA(B2) mRNA levels. Moreover, GABA(B1) and GABA(B2) receptor mRNA levels were increased on 3-week ethanol treatment. Finally, GABA(B) agonist baclofen and antagonist phaclofen showed significant decreasing effects on GABA(B1) receptor mRNA levels in the cortex, but not in the hippocampus. These results were further confirmed by in situ hybridization. Thus, the present results showed the effects of ethanol on GABA(B) receptors in the cortex and hippocampus, implying the possible role of GABA(B) receptor in ethanol effects. The effects of GABA(B) receptor agonist and antagonist suggested that the possible mechanisms underlying that GABA(B) receptor modulated the behavioral effect induced by ethanol. |
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Keywords: | CA1, field CA1 in hippocampus CA2, field CA2 in hippocampus CA3, field A3 in hippocampus CREB, cAMP-response element binding protein DG, dentate gyrus GABA, gamma-aminobutyric acid DEPC, diethyl pyrocarbonate 4% NBP, 4% paraformaldehyde in 0.1 M sodium phosphate RPA, RNase protection assay SSC, sodium chloride-sodium citrate |
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