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| SARS冠状病毒PUMC2株全基因组cDNA分段克隆 | |
| 引用本文： | Fan Z,Tan XY,Yin B,Zou K,Wang T,Shen Y,Ni AP,Qin C,Yuan JG,Qiang BQ,Peng XZ. SARS冠状病毒PUMC2株全基因组cDNA分段克隆[J]. 中国医学科学院学报, 2003, 25(5): 499-503 |
| 作者姓名： | Fan Z Tan XY Yin B Zou K Wang T Shen Y Ni AP Qin C Yuan JG Qiang BQ Peng XZ |
| 作者单位： | 1. 中国医学科学院,中国协和医科大学,基础医学研究所医学分子生物学国家重点实验室,北京,100005 2. Chinese National Human Genome Center, Beijing 3. Department of Clinical Laboratory, PUMC Hospital, CAMS and PUMC,Beijing 4. Institute of Experimental Animal, CAMS and PUMC, Beijing |
| 摘 要： | 目的 分段克隆SARS冠状病毒PUMC2株的全基因组cDNA。方法 以SARS冠状病毒PUMC2株基因组RNA为模板,用RT-PCR扩增cDNA片段,PCR产物经纯化后,连接人pGEM-T载体中,进行序列测定。结果 获得了SARS冠状病毒PUMC2株基因组全长cDNA的分段克隆。结论 SARS冠状病毒PUMC2株基因组全长cDNA分段克隆的获得,为SARS冠状病毒基因功能的研究和全长有感染性cDNA的克隆奠定了基础。 |
| 关 键 词： | SARS冠状病毒PUMC2株 分段克隆 |
| 修稿时间： | 2003-08-07 |
cDNAs cloning of SARS-CoV PUMC2 viral genome |
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| Fan Zheng,Tan Xin-yu,Yin Bin,Zou Ke,Wang Ting,Shen Yan,Ni An-ping,Qin Chuan,Yuan Jian-gang,Qiang Bo-qin,Peng Xiao-zhong. cDNAs cloning of SARS-CoV PUMC2 viral genome[J]. Acta Academiae Medicinae Sinicae, 2003, 25(5): 499-503 | |
| Authors: | Fan Zheng Tan Xin-yu Yin Bin Zou Ke Wang Ting Shen Yan Ni An-ping Qin Chuan Yuan Jian-gang Qiang Bo-qin Peng Xiao-zhong |
| Affiliation: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China. |
| Abstract: | OBJECTIVE: To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain. METHODS: Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced. RESULTS: The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained. CONCLUSIONS: These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV. |
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