| 高三尖杉酯碱对HL-60细胞端粒酶量的抑制效应 |
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| 引用本文： | 谢万灼,林茂芳. 高三尖杉酯碱对HL-60细胞端粒酶量的抑制效应[J]. 中国药理学与毒理学杂志, 2002, 16(1): 31-36 |
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| 作者姓名： | 谢万灼 林茂芳 |
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| 作者单位： | 浙江大学医学院附属第一医院血液科,浙江,杭州,310003 |
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| 摘 要： | 目的 研究高三尖杉酯碱 (HHT)对HL 6 0细胞端粒酶活性的影响及诱导凋亡作用。方法 采用端粒重复序列扩增法 (TRAP) 酶联免疫吸附试验(ELISA)检测了HL 6 0细胞的端粒酶量变化 ,用细胞形态学观察 ,DNA琼脂糖凝胶电泳 ,流式细胞术检测和DNA片段原位末端标记 (TUNEL)法检测了细胞凋亡现象。结果 5～ 5 0 0 μg·L- 1HHT处理HL 6 0细胞 0～ 4 8h ,HL 6 0细胞端粒酶量呈剂量依赖性和时间依赖性下降。同时 ,HL 6 0细胞发生了凋亡。结论 HHT有降低HL 6 0细胞的端粒酶量 ,诱导细胞凋亡作用。
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| 关 键 词： | 高三尖杉酯碱 端粒,末端转移酶 白血病,细胞 凋亡 |
| 收稿时间： | 2000-12-12 |
Inhibitory effect of homoharringtonine on telomerase content of HL-60 cells |
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| XIE Wan-Zhuo, LIN Mao-Fang. Inhibitory effect of homoharringtonine on telomerase content of HL-60 cells[J]. Chinese Journal of Pharmacology and Toxicology, 2002, 16(1): 31-36 |
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| Authors: | XIE Wan-Zhuo LIN Mao-Fang |
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| Affiliation: | (The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China) |
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| Abstract: | AIM To explore the effect of homoharringtonine (HHT) on telomerase content of HL- 60 cells and HHT-induced apoptosis. METHODS The telomerase content of HL- 60 cells was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphological observation, DNA agarose gel elec trophoresis, flow cytometry (FCM) and TdT-mediated dUTP-biotin nick end labelling (TUNEL). RESULTS With HL-60 cells being treated with HHT at 5－500 μg·L-1 for 48 h, the level of telomerase content in HL- 60 cells decreased in a concentration- and time-dependent manner. Simultaneously, HL-60 cells underwent apoptosis. CONCLUSION HHT can decrease the telomerase content of HL 60 cells effectively an d induce apoptosis. |
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| Keywords: | homoharringtonine telomerase leukemia cell apoptosis |
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