青蒿琥酯抑制新生血管生成的作用

目的　为研究青蒿琥酯成为抗血管生成药物的可能性 ,观察其对新生血管的影响 ,并初步探讨其机制。方法　采用鸡胚绒毛尿囊膜、大鼠主动脉环无血清培养、人脐静脉内皮细胞损伤迁移等实验 ,检测青蒿琥酯对新生血管增殖与迁移的抑制作用。结果　鸡胚绒毛尿囊膜实验表明 ,青蒿琥酯有较强的血管增殖抑制作用 ,对微血管作用强于大血管 ;大鼠主动脉环无血清培养实验表明 ,青蒿琥酯能明显推迟血管新生 ,减少新生血管数量 ;人脐静脉内皮细胞损伤迁移实验表明青蒿琥酯对内皮细胞具有增殖和迁移抑制作用。在这些体内外实验中 ,青蒿琥酯抑制血管生成作用呈剂量依赖性。结论　青蒿琥酯具有抑制新生血管生成作用 ,此作用可能与抑制血管内皮细胞迁移有关。

Inhibitory effect of artesunate on angiogenesis

AIM To study whether artesunate may be used as an anti angiogenetics. METHODS The inhibition of artesunate on angiogenesis was studied in the model of chick chorioallantoic membrane, the culture of rat aortic sprouting in fibrin gel, as well as wound assay of human umbilical vein endothelial cell(HUVEC). RESULTS Artesunate significantly inhibited chick chorioallantoic membrane angiogenesis, the effect on microvessels was stronger than on main vessels. Rat aortic sprouting in fibrin gel experiment showed that artesunate inhibited microvessel sprouting and put off microvessel forming. Wound assay for migration of HUVEC showed that artesunate inhibited HUVEC proliferation and migration. Both experiments in vivo and in vitro showed dose dependent inhibition on angiogenesis and migration by artesunate. CONCLUSION Artesunate can inhibit angiogensis, which may be related to the decreased migration of endothelial cell.