改良蛋氨酸胆碱缺乏饮食喂养的非酒精性脂肪性肝炎大鼠模型的建立

目的:观察改良蛋氨酸胆碱缺乏饮食(MCD)建立的脂肪性肝炎大鼠模型肝脏的脂肪变性和炎症情况.方法:大鼠26只随机分为4组,分别喂养MCD饮食和胆碱添加饮食(CS).3-8wk后处死,肝脏标本在甲醛中固定和石蜡包埋.HE染色和Masson染色后对脂肪变性、炎症和纤维化进行评分.血清ALT,AST,CH,LDL等指标通过生化分析仪进行测定.结果:MCD喂养组3wk可以看见肝脂肪变性和炎症,肝指数显著增加,ALT和AST显著升高,8wk可见纤维形成.结论:可通过改良的蛋氨酸胆碱缺乏(MCD)饮食建立非酒精性脂肪肝动物模型.

Establishment of nonalcoholic steatohepatitis rat models with modified methionine-choline-deficient diets

AIM:To establish nonalcoholic steatohepatitis(NASH)rat models with modified methionine-choline-deficient(MCD)diets.Hepatic steatosis and inflammation would be expected in these rats.METHODS:Rats were randomly divided into 4 groups.Rats were fed with MCD or with choline-supplemented(CS)diets.Weight and food intake were determined.After 3 and 8 weeks,rats were sacrificed;liver specimens were fixed overnight in buffered formaldehyde(40 g/L)and embed-ded in paraffin.HE-stained and Massion-stained sections were graded blindly for the degree of fatty change,inflammation,fibrosis and necrosis.Serum was measured for biochemical markers.RESULTS:After 3 weeks on an MCD diet,ste-,atosis and inflammation could be seen in rat liv-ers.The liver index was increased significantly by the MCD diet.Serum ALT and AST levels were also increased significantly,whereas TG levels decreased.After 8 weeks on the diet,fi-brogenesis could be seen.CONCLUSION:Rat NASH models can be estab-lished using modified MCD diets.