不同级别胃黏膜上皮瘤变组织TLR4表达与HP感染的相关性研究

目的：观察低级别和高级别上皮瘤变组织和胃癌组织TLR4的表达和HP感染的关系，探讨两者的相关性。方法：应用免疫组化方法检测30例正常胃黏膜，30例低级别上皮内瘤变组织，30例高级别上皮内瘤变组织及30例胃癌组织中的TLR4的表达。利用^14C幽门螺杆菌呼气试验及改良Giemsa染色法检测HP，两项均阳性者诊断为HP感染。结果：TLR4在低级别上皮内瘤变组的阳性表达率为90．0％，高级别上皮内瘤变组为86．7％，胃癌组63．3％，正常组26．7％，HP阳性率在低级别上皮内瘤变组为93．3％，高级别上皮内瘤变组90％，正常组33．3％；低级别上皮内瘤变组、高级别上皮内瘤变组、胃癌组TLR4阳性率与正常组比较，P〈0．05，低级别上皮内瘤变组、高级别上皮内瘤变组TLR4阳性率与胃癌组比较，P〈0．05。TLR4表达与HP感染检测结果：HP感染的胃黏膜组织TLR4的阳性表达率为83．53％，HP阴性组TLR4阳性表达率为25．71％，TLR4表达和HP感染具有相关性r=0．152，P=0．012。HP感染与不同级别胃黏膜上皮瘤变组织TLR4表达的相关性检测结果：低级别胃黏膜组织11LR4的阳性表达率为88．89％，高级别胃黏膜组织为88．00％。胃黏膜上皮组织瘤变级别与TLR4表达无相关性，P〉0．05。结论：低级别上皮瘤变和高级别上皮瘤变胃黏膜组织TLR4的阳性表达明显高于胃癌组织；HP感染和TLR4阳性表达呈正相关，HP感染的不同级别胃黏膜上皮瘤变组织之间TLR4表达无相关性。

Association between TLR4 expression and Helicobacter pylori infection in different grades of gastric mucous intraepithelial neoplasia tissues

Objective：To observe TLR4 expression and its association with Helicobacter pylori （ H. pylori ） infection in high and low grades gastric mucous intraepithelial neoplasia tissues and gastric cancer tissues. Methods：Thirty normal gastric mucous tissues, 30 low grade intraepithelial neoplasia tissues, 30 high grade intraepithelial neoplasia and gastric cancer tissues were examined by immunohistochemistry method for TLR4 expression . ^14C expiratory test and improved Giemsa dyeing method were used to examine H. pylori. Results： Positive rate of TLR4 in low grade intraepithelial neoplasia group was 90.0%, in high grade intraepithelial neoplasia group was 86.7%, in gastric cancer group was 63.3% ,in normal gastric mucous group was 26.7%. Positive rate of H. pylori in low grade intraepithelial neoplasia group was 93.3% ,in high grade intraepithelial neoplasia group was 90%, in normal gastric mucous group was 33.3%. Compared to normal gastric mucous group,positive expression rate of TLR4 in low and high grade intraepithelial neoplasia groups and gastric cancer group P 〈 0.05. Compared to gastric cancer group, positive expression rate of TLR4 in low and high grade intraepithelial neoplasia groups P 〈 0.05. Positive expression rate of TLR4 in H. pylori positive group was 83.53% ,in H. pylori negative group was 25.71%. TLR4 expression was associated with H. pylori infection P 〈 0.05. Positive expression rates of TLR4 in low and high grade mucous tissue were respectively 88.89% ,88.00%. TLR4 expression had no obvious association with grades of gastric mucous intraepithelial neoplasia,P 〉 0. 05. Conclusion：Positive expression rates of TLR4 in low and high grades intraepithelial neoplasia tissues are obviously higher than in gastric cancer tissues. H. pylori infection has positive association with expression of TLR4, no association in different grades of gastric mucous intraepithelial neoplasia tissues with H. pylori infection.