自身免疫性溶血性贫血患者的血型检定研究

为了观察自身抗体对ABO和RhD血型检定的干扰，对38例自身免疫性溶血性贫血(autoimmune hemolytic anemia，AIHA)患者血液标本进行ABO和RhD常规定型，氯奎放散试验后定型及基因定型。结果表明：38例AIHA患者中，ABO血型难定者11例(31.6％)，均为间接抗球蛋白试验阳性及正反定型不合。RhD(-)误定RhD( )1例，并含抗-D。采用氯奎放散试验后血型皆正确检定。结论：AIHA患者的自身抗体干扰血型检定，必须采用多种技术才能正确检定血型，确保安全输血。

Study on Blood ABO Typing in Patients with Autoimmune Hemolytic Anemia

To explore effect of autoantibody on identification of ABO and RhD blood group,the blood samples of 38 patients with antoimmune hemolytic anemia (AIHA) were identified by routine typing and typing after chloroquine elution test as well as PCR. The results showed that out of 38 patients with AIHA,11 cases (31.6%) of ABO blood group were difficultly typed,indirect antiglobulin test were positive,and contradiction between cells typing and sera typing were observed. 1 case of RhD(-) was mistyped as RhD( ) and anti-D was found in its serum. The blood group of these cases were typed correctly by chloroquine elution test. It is concluded that blood group identification of patients with AIHA can be interefered by autoantibody,and the correct typing for blood group of these patients may be determined by using combination of several methods to ensure safe transfusion.