丙型肝炎病毒核心抗原检测的临床应用探讨

目的]探讨HCV核心抗原在HCV感染诊断和治疗中的作用。方法]对173例HCV感染患者血清和82例健康对照者血清,使用ELISA法分别检测抗-HCV、总的HCV核心抗原,使用荧光定量RT-PCR检测HCV RNA。结果]173例HCV感染者抗-HCV均为阳性。HCV RNA阳性率为49.7%(86/173);HCV核心抗原阳性率为36.4%(63/173)。二者差异有统计学意义(P<0.05)。86例HCV RNA阳性标本中,HCV核心抗原阳性60例(69.8%);HCV核心抗原阴性26例(30.2%)。82例健康对照者抗-HCV、HCV核心抗原、HCV RNA均为阴性。结论]在没有HCV RNA检测资格的实验室,HCV核心抗原检测可作为病毒存在和复制的一个指标,作为抗-HCV检测的补充试验。

Clinical significance of testing hepatitis C virus core antigen

Objective]To investigate the significance of serum total HCV core antigen(HCV-cAg) in the HCV infection and treatment.Methods]One hundred and seventy-three serum specimens of HCV infection patients were examined for HCV cAg and anti-HCV antibody with ELISA,for HCV RNA with real-time RT-PCR.Results]In 173 anti-HCV positive specimens,the positive rate of HCV RNA was 49.7%(86/173) and the positive rate of HCV-cAg was 36.4%(63/173).The positive rate of HCV RNA was higher than that of HCV-cAg(P<0.05).In 86 HC...