大鼠睾丸组织中ALR的研究

目的:研究大鼠睾丸组织中肝再生增强因子(ALR)的表达情况,探讨睾丸ALR与生殖的关系.方法:取幼年、成年、老年大鼠睾丸组织以及部分肝切除(PH)术前、术后12h、24h、48h大鼠肝脏、睾丸组织进行免疫组化,了解大鼠睾丸组织中ALR的表达.结果:睾丸组织中ALR广泛存在于间质细胞、支持细胞和生精细胞,其中尤以精原细胞、精母细胞表达最强.不同年龄段ALR的表达不一样,幼年最强、成年次之、老年最弱.70%PH后肝脏ALR表达增强,24h达峰值,睾丸组织中ALR表达无明显变化.结论:睾丸组织有较强ALR表达,不同脏器的ALR作用具有脏器特异性,ALR与睾丸的发育以及精子生成及成熟密切相关.

Study of augmenter of liver regeneration in rat testis

Objective:To confirm the expression and localization of augmenter of liver regeneration(ALR) in baby,mature and the old rat testis and to evaluate the function of ALR in reproduction.Methods:ALR expression and localization in baby,mature and the old rat testis were examined by immunohistochemistry,ALR expressions of the mature rat testis and liver were examined in 12h,24h and 48h after 70 percent partial hepatectomy(PH) as well.Results:ALR was expressed in the cytoplasm and membrane of cells and it commonly existed in Leydig cells,Sertoli cells and sperm cells of rat testis,especially in spermatogonia and spermatocytes.ALR expressions differed among baby,mature and the old rat testis,in the way from the strongest to the weakest.After 70 percent PH ALR expression increased in liver,peaking in 24h,while that in testis didn't change.Conclusion:ALR expression in testis is strong which is necessary for testis development,spermatogenesis and mature sperm.The function of ALR is organ special.