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锌指蛋白基因对内毒素诱导选择素表达的影响
引用本文:糜建红,朱楚洪,应大君.锌指蛋白基因对内毒素诱导选择素表达的影响[J].解剖学报,2004,35(5):548-550.
作者姓名:糜建红  朱楚洪  应大君
作者单位:第三军医大学解剖学教研室,生物力学与组织工程学教育部重点实验室,生物力学研究室,重庆,400038
基金项目:全军十五青年基金资助项目 ( 0 1Q0 98),教育部生物力学及组织工程重点实验室访问学者基金资助项目,重庆市科学基金资助项目 ( 2 0 0 10 60 )
摘    要:目的 探讨外源性A2 0基因对内毒素诱导的内皮细胞E 选择素表达的影响。 方法 DOTAP脂质体介导的pCNDA3 1EHA2 0基因转染人脐静脉内皮细胞 ,经G4 18筛选阳性克隆 ,免疫荧光检测A2 0基因的表达 ,原位杂交、免疫荧光及Westernblot分别检测内皮细胞E 选择素的表达。 结果 A2 0基因在经G4 18筛选后的内皮细胞中有高表达 ;内毒素能诱导人内皮细胞E 选择素表达 ;A2 0基因能抑制内毒素诱导的内皮细胞 90 %的E 选择素表达 ,两者间相差显著 (P <0 0 5 )。 结论 A2 0基因能够显著抑制内毒素诱导的内皮细胞活化 ,有助于创伤的治疗。

关 键 词:A20基因  E-选择素  内毒素

OVEREXPRESSION OF ZINC FINGER PROTEIN INHIBITS LPS-INDUCED EXPRESSION OF E-SELECTIN IN ENDOTHELIAL CELLS
MI Jian-hong,ZHU Chu-hong,YING Da-jun.OVEREXPRESSION OF ZINC FINGER PROTEIN INHIBITS LPS-INDUCED EXPRESSION OF E-SELECTIN IN ENDOTHELIAL CELLS[J].Acta Anatomica Sinica,2004,35(5):548-550.
Authors:MI Jian-hong  ZHU Chu-hong  YING Da-jun
Institution:MI Jian-hong,ZHU Chu-hong,YING Da-jun *
Abstract:Objective Previous studies have demonstrated that LPS can induce endothelial cell activation and the expression of E-selectin. In this study, we examined whether A20 gene could inhibit the expression of E-selectin in endothelial cells induced by LPS. Methods With the help of DOTAP, endothelial cells were transfected with pCDNA3.1 EHA20. The postive cell clones were selected with G418.The stable transfection and expression of A20 in the endothelial cells were determined by immunofluorescence analysis. The E-selectin expression was checked by immunofluorescence, Western blot and in situ hybridization. Results Abundant A20 stable expression in endothelial cells transfected with pCDNA3.1 EHA20 was confirmed by immunofluorescence analysis. E-selectin expression increased in LPS-inducible endothelial cells. A20 gene inhibited 90% LPS-inducible E-selectin expression(P<0.05). Conclusion With the help of DOTAP,hA20 gene can be transferred and stably expressed in endothelial cells and it can inhibit LPS-induced E-selectin expression of endothelial cells.
Keywords:A20 gene  E-selectin  Lipopolysaccharide(LPS)
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