Production of lymphokines and interferon by immune cells involved in recovery of mice from herpes simplex virus type 2 hepatitis |
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Authors: | D Iannello S C Mogensen |
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Institution: | 1. Institute of Medical Microbiology, University of Aarhus, Aarhus, Denmark |
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Abstract: | Adoptive transfer of spleen cells from mice 4 days after infection with herpes simplex virus type 2 (HSV-2) reduced the virus titer in the liver of recipient mice infected 24 h before transfer. Macrophage chemotactic factor (CF) and macrophage migration inhibition factor (MIF) were produced by day 3 of infection in spleen cell cultures stimulated with HSV-2, but not with control antigen, i.e. 1 day before the cells are active in adoptive transfer. Interferon was produced in cultures established throughout the infection but not in normal spleen cells. From days 1 to 5 of infection interferon was produced irrespective of in vitro restimulation, although the highest amounts were always produced after stimulation with the specific antigen. Spleen cells from mice infected for 6 days produced interferon only when stimulated with HSV-2. The cells from 6-day-immune mice active in adoptive transfer and CF and MIF production were found to be Thy 1+, Ig- and Lyt2-. Both Thy 1+ and plastic adherent cells were necessary for interferon production, whereas Ig+ and Lyt2+ cells did not produce interferon. The interferon was acid stable and neutralized by antiserum against alpha/beta-interferon and thus has the characteristics of alpha-interferon. The data indicate that a delayed type hypersensitivity reaction with lymphokine-induced macrophage recruitment into infectious foci may be a central feature of the recovery process in HSV-2-induced hepatitis. A possible role of interferon produced by the accumulated cells needs further investigation. |
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Keywords: | HSV-2 herpes simplex virus type 2 HSV-1 herpes simplex virus type 1 NK cells natural killer cells M45 macrophages DTH delayed type hypersensitivity MHC major histocompatibility complex CF chemotactic factor CI chemotactic index MIF migration inhibition factor MI migration index ISC immune spleen cells i v intravenous i p intraperitoneal PFU plaque-forming units FCS fetal calf serum |
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