CD44v6与乳腺癌转移和预后的关系

目的　研究CD44v6表达与乳腺癌转移和预后的关系。　方法　用免疫组化方法对 78例乳腺癌石蜡标本及 37例乳腺癌新鲜标本的CD44v6蛋白的表达水平进行了检测 ;RT PCR方法对 37例乳腺癌新鲜标本的CD44v6mRNA表达水平进行了检测。并对患者CD44v6的表达水平、肿瘤大小、腋淋巴结转移状况、TNM分期、组织学分级与预后间的关系进行了分析 ,同时比较 2种方法的敏感性。　结果　乳腺癌患者的CD44v6表达水平与原发肿瘤大小、腋淋巴结转移状况、TNM分期明显相关 ,是影响患者术后 5年生存率的重要因素。乳腺癌新鲜标本中CD44v6mRNA和蛋白的阳性表达率分别是 70 3%和 5 9 5 % ,两者差异无显著性意义 (P >0 0 5 )。　结论　乳腺癌患者的CD44v6的表达水平与癌细胞的转移和患者的预后相关 ;免疫组化和RT -PCR 2种方法 ,在检测CD44v6的敏感性方面差异无显著性。

The relationship of expression of CD44v6 with metastasis and prognosis in breast cancer

OBJECTIVE: To study the relationship of expression of CD44v6 with metastasis and prognosis in breast cancer. METHODS: The expression of CD44v6 protein in 78 paraffin and 37 fresh specimens of breast carcinomas was detected with immunohistochemistry (IHC). The expression of CD44v6 mRNA in 37 fresh specimens of breast carcinoma was detected with RT-PCR. The correlation of tumor size, TNM stage, status of axillary lymph node, tissue grade, prognosis and CD44v6 expression in breast cancer was analyzed to compare the sensitivity of the two methods. RESULTS: The expression of CD44v6 in breast cancer was correlated to metastasis of the axillary lymph node, tumor size and TNM stage. It was an important factor affecting survival of patients with breast cancer. Positive expressive rates of CD44v6 protein and mRNA were 70.3% and 59.5% respectively in 37 specimens of fresh carcinoma tissues (P > 0.05). CONCLUSIONS: The expression of CD44v6 is closely related to metastasis and prognosis in breast cancer, and the sensitivity to detect CD44v6 expression shows no significant difference between IHC and RT-PCR.