Effects of esculentoside A on production of interleukin-1, 2, and prostaglandin E2 |
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Authors: | Xiao Zhen-Yu Zheng Qin-Yue Jiang Yuan-Ying Zhou Bin Yin Ming Wang Hong-Bin Zhang Jun-Ping |
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Institution: | [1]DepartmentofPharmacology,SecondMilitaryMedicalUniversity,Shanghai200433 [2]CollegeofPharmacy,JiaoTongUniversity,Shanghai200030,China |
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Abstract: | AIM: To investigate the influence of esculentoside A (EsA) on immunological function and its mechanism of anti-inflammation. METHODS: Interleukin-1 production was measured by thymocyte co-stimulating assay; the radioactivity of (3)H]arachidonic acid (AA) was used to evaluate the release of AA; prostaglandin E2 production was measured with radioimmunoassay (RIA); IL-2 and IFN-gamma were detected by ELISA method. RESULTS: EsA (3-12 micromol/L)could potently inhibit the production of IL-1 and PGE(2) from both silent and LPS induced macrophages. EsA had no significant effect on the release of AA from murine macrophages. EsA could inhibit the production of IL-2 from murine lymphocytes induced by ConA, but not affect the production from silent lymphocytes. EsA showed no effect on the production of IFN-gamma from both silent and ConA induced lymphocytes. CONCLUSION: EsA could affect the immunological function through inhibiting the production of IL-2 from activated splenocytes and the inhibition of production of IL-1 and PGE(2) might be one of the anti-inflammation mechanisms of EsA. |
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Keywords: | esculentoside A interleukin-1 interleukin-2 prostaglandins E interferon-gamma |
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