酸枣仁汤对失眠小鼠血清、肝脏和回肠中胆汁酸分子水平的调控作用

为了从内源性胆汁酸调控角度探讨酸枣仁汤疏肝治疗失眠的机制,对酸枣仁汤护肝作用、失眠模型小鼠及酸枣仁汤治疗后小鼠血清、肝脏和回肠中胆汁酸分子水平变化进行考察.采用睡眠剥夺法建立小鼠失眠模型,灌胃给予酸枣仁汤(48.96 mg·kg-1 ·d-1)7 d后,对各组小鼠基本药效指标饮水饮食量、体质量以及谷草转氨酶(AST)、...

Effect of Suanzaoren Decoction on molecular levels of bile acids in serum,liver,and ileum of insomnia mice

To explore the mechanism of Suanzaoren Decoction in the treatment of insomnia from endogenous bile acid regulation,the present study investigated the hepatoprotective effect of Suanzaoren Decoction and the molecular changes of bile acids in the serum,liver,and ileum of insomnia model mice and Suanzaoren Decoction treated mice.The insomnia model in mice was established by the sleep deprivation method.After Suanzaoren Decoction(48.96 mg·kg^-1·d^-1)intervention by gavage for 7 days,the related indicators,such as water consumption,food intake,body weight,aspartate aminotransferase(AST),alanine transaminase(ALT),and total bile acid(TBA)were detected,and the pathological changes of the liver and ileum were observed.The molecular levels and distribution of 23 bile acids in the serum,liver,and ileum were analyzed by UPLC-MS/MS combined with principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA).The results showed that Suanzaoren Decoction could improve the decreased water consumption and food intake,weight loss,and increased AST and ALT in the model group,and effectively reverse the injury and inflammation in the liver and ileum.The bile acids in the liver of the insomnia model mice were in the stage of decompensation,and the bile acids in the serum,liver,and ileum of the mice decreased or increased.Suanzaoren Decoction could regulate the anomaly of some bile acids back to normal.Seven bile acids including glycoursodeoxycholic acid(GUDCA),glycodesoxycholic acid(GDCA),tauro-α-MCA(T-α-MCA),α-MCA,taurodeoxycholate(TDCA),T-β-MCA,and LCA were screened out as the main discriminant components by PLS-DA.It is concluded that Suanzaoren Decoction possesses the hepatoprotective effect and bile acids could serve as the biochemical indicators to evaluate the drug efficacy in the treatment of abnormal liver functions caused by insomnia.The mechanism of Suanzao-ren Decoction in soothing the liver,resolving depression,tranquilizing the mind,and improving sleep may be related to the molecular regulation of bile acid signals.