血清氯化物酶法测定

目的建立血清氯化物的α-淀粉酶及其底物2-氯-4-硝基苯-α-D-麦芽三糖苷(CNP-G3)直接测定方法。方法根据氯离子是α-淀粉酶的激动剂原理建立氯化物测定方法,并做方法学评价。结果测定结果的平均偏差率为0.46%;线性范围50~150mmol/L;平均回收率为100.62%;批内CV<1.24%,日间CV<1.86%,总CV1.45%~2.24%;加入高浓度的维生素C、葡萄糖、蔗糖、果糖、α-淀粉酶、三酰甘油、血红蛋白、胆红素、钙离子、溴离子、碘离子、氟离子、硫氰酸钾、对硝基酚、叠氮钠和硫酸铵均未显示干扰。与硫氰酸汞比色法(MTC)(x±s)比较:MTC法(x):(95.66±14.45)mrnol/L,酶法(Y):(95.62±14.93)mmol/L,t=0.078,P>0.5,Y=1.016X-1.587,r=0.984,n=93,P<0.001;与离子选择电极法(ISE)(x±s)比较:ISE法(X):(99.00±13.11)mmol/L,本法(Y)(98.61±12.70)mmol/L,t=0.140,P>0.5,Y=0.962X+3.375,r=0.993,n=44,P<0.001。溶解的工...

Direct enzymatic determination of chloride in serum

Objective To set up a new direct measuring method for serum chloride by usingα-amylase and its substrate 2-chloro-4-nitrophenyl-alpha-maltotrioside.Methods The principle of the proposed method was according to chloride being a stimulator for α-amylase.Results The mean bias between assay values and target values in quality control sera was 0.46%.The linearity range was from 50 mmol/L to 150 mmol/L.The mean recovery rate was 100.62%.The coefficient of variation(CV) of within runs was less than 1.24%.The CV of...