小鼠树突状细胞肉瘤细胞系的建立及形态学鉴定

本研究目的是建立鉴定树突状细胞肉传代细胞系，即荷ＬＩＩ瘤６１５鼠脾脏进行原代细胞培养，经传统代培养后新建立一株体外传代细胞系，经鉴定为树突状细胞肉瘤细胞系，命名为ＤＣＳ。该细胞体外呈分支状。酶组织化学检查发现碱性磷酶酶、酸性磷酶酶、１非特异性脂酶、过氧化酶及胞膜ＡＴＰａｓｅ均阴性。

ESTABLISHMENT AND IDENTIFICATION OF A DENDRITIC CELL SARCOMA CELL LINE (DCS): I CYTOLOGY, HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY

DCS cells has been established from splenic tumor of a mouse. The cells is adherent, dendritic, and its cytoplasm has some global mitochondria but few phagolysosomes. DCS does not express differentiation antigens of monocytes, lymphocytes. We used the polyclonal antibodies produced from rabbits against DCS cells to find the original cells in mouse spleen. The positive cells was in the T areas, i.e. the periarteriolar lymphoid sheaths(PAL), and in the skin the positive cells was Langerhans cells. Among adherent splenic cells there were some cells, which did not phagocytose particles and also did not pinocytose HRP, but could be stained by the serum against DCS with the routine ABC method. All cells of DCS was peroxidase negative, acid phosphatase negative,alkaline phosphatase weak or negative,non specific esterases negative and membrane ATPase negative. It is considered that the DCS cells originated from interdigital dendritic cells of mouse spleen.