Hirschsprung's disease: a comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro |
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| Authors: | L T Larsson G Malmfors C Wahlestedt S Leander R H?kanson |
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| Affiliation: | 1. Department of Biotechnology, Regional Centre for Biotechnology, Faridabad, Haryana, India;2. Department of Microbiology, Institute of Home Economics, University of Delhi, New Delhi, India;3. Department of Microbiology, Gargi College, University of Delhi, New Delhi, India;1. Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;2. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran;3. Bioinformatics Research Group, Mashhad University of Medical Sciences, Mashhad, Iran;4. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;5. Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;2. Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology, Parkville, VIC, Australia;3. The University of Melbourne, Parkville, VIC, Australia |
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| Abstract: | Specimens from aganglionic (constricted) and ganglionic (dilated) gut were obtained from nine patients with Hirschsprung's disease. Transmural nerve stimulation of ganglionic smooth muscle in vitro evoked an initial relaxation followed by a contraction. This contraction was reduced but not abolished by atropine and it was further reduced by substance P antagonists. Guanethidine did not affect the electrically evoked responses. In aganglionic smooth muscle, an atropine-sensitive contraction but no initial relaxation was registered. Tetrodotoxin abolished all responses to electrical stimulation in both ganglionic and aganglionic specimens. Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens. Two other gut neuropeptides, neuropeptide Y and galanin, were without effect. The results do not indicate a different receptor set up in ganglionic v aganglionic gut. The results are compatible with a lack of noncholinergic nonadrenergic inhibitory neurons in the aganglionic gut. |
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