Identification of plant extracts with potential antidiabetic properties: effect on human peroxisome proliferator‐activated receptor (PPAR), adipocyte differentiation and insulin‐stimulated glucose uptake |
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| Authors: | Kathrine B. Christensen Ariane Minet Henrik Svenstrup Kai Grevsen Hongbin Zhang Eva Schrader Gerald Rimbach Silvia Wein Siegfried Wolffram Karsten Kristiansen Lars P. Christensen |
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| Affiliation: | 1. Department of Food Science, University of Aarhus, Kirstinebjergvej 10, 5792 Aarslev, Denmark;2. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark;3. Department of Horticulture, University of Aarhus, Kirstinebjergvej 10, 5792 Aarslev, Denmark;4. Department of Human Nutrition and Food Science, Christian‐Albrechts University, Hermann‐Rodewald‐Str. 6, 24098 Kiel, Germany;5. Department of Animal Nutrition and Physiology, Christian‐Albrechts University, Hermann‐Rodewald‐Str. 9, 24098 Kiel, Germany;6. Department of Biology, University of Copenhagen, Ole Maal?es Vej 5, 2200 Copenhagen N, Denmark;7. Institute of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Niels Bohrs Allé 1, 5230 Odense M, Denmark |
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| Abstract: | Thiazolidinediones (TZDs) are insulin sensitizing drugs used to treat type 2 diabetes. The primary target of the TZDs is the peroxisome proliferator‐activated receptor (PPAR) γ, a key regulator of adipogenesis and glucose homeostasis. Currently prescribed TZDs are full PPARγ agonists, and their use is associated with several side effects. Partial PPARγ agonists appear to be associated with fewer side effects but may still confer the desired insulin sensitizing action. Extracts from common medicinal/food plants were tested in a screening platform comprising a series of bioassays, including tests for PPARγ, α and δ transactivation, adipocyte differentiation and insulin‐stimulated glucose uptake, allowing identification of plants containing potentially interesting PPAR agonists. Twenty‐two plant extracts out of 133 were found to increase insulin‐stimulated glucose uptake and 18 extracts were found to activate PPARγ, 3 to activate PPARα and γ, 6 to activate PPARδ and γ, and 9 to activate PPARγ, α and δ. Among the 24 different plant species tested in the platform, 50% were shown to contain compounds capable of activating PPARγ and stimulating insulin‐dependent glucose uptake with no or little effect on adipocyte differentiation warranting further studies and characterization. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | medicinal plants food plants type 2 diabetes peroxisome proliferator‐activated receptors (PPARs) adipocyte differentiation glucose uptake |
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