MDR1/P-gp在膀胱移行细胞癌中的表达及与Fas和Survivin蛋白表达的关系

目的：探讨膀胱移行细胞癌中MDR1基因（P-糖蛋白）与Fas、Survivin表达的相互关系及其与膀耽癌生物学行为的相关性。方法：应用免疫组织化学方法检测64例膀耽移行细胞癌和12例正常膀胱粘膜中P—GP、Fas、Survivin的表达。结果：膀胱移行细胞癌中P—GP、Survivin的表达阳性率高于正常粘膜，与膀胱癌的分级有关（P〈0．01）;而Fas在膀胱正常粘膜中表达高于移行细胞癌，差异有统计学意义（P〈0．01）复发性膀胱癌P-GP的阳性表达率高于初发膀胱癌（P〈0．01）、膀胱移行细胞癌P—GP的表达与Fas呈负相关．而与Survivin表达无关.结论：膀胱移行细胞癌的MDR1（多药耐药基因）与Fas的表达密切相关．而与凋亡抑制蛋白Sureivin的表达无关.本研究为采用MDR逆转剂或Fas干扰剂以增加膀胱移行细胞癌的化疗敏感性提供实验依据。

Correlations between the Expression of MDR1/P-glycoprotein,Survivin and Fas in Transitional Cell Carcinoma of the Bladder

Objective:To explore the interrelation between expression of MDR1/P-glycoprotein,Fas and Survivin and to study the correlation between these expression levels and the biological behavior of transitional cell carcinoma of the bladder (TCCB).Methods:Immunohistochemistry was used to detect the expression of P-GP,Survivin and Fas in TCCB (n=64) and normal bladder mucosa (n=12).Results:The expression level of P-GP and Survivin in TCCB was higher than those levels found in normal bladder mucosa (P<0.01).The expression levels of P-GP and Survivin were strongly correlated with clinical stage (P<0.01).Fas was detected in 50% of the TCCB cases and in 100% of the normal bladder mucosa samples (P<0.01).P-GP expression in recurrent TCCB was higher than in primary TCCB (P<0.01).The expression of P-GP in TCCB had a negative correlation with that of Fas,but had no correlation with Survivin expression.Conclusions:Multiple drug resistance in TCCB is strongly correlated with the expression of P-GP and Fas,but not with Survivin expression.Our experimental evidence suggests that chemosensitivity may be increased by using MDR reversing agents or Fas interfering agents.