毛细管气相色谱法测定醒脑静葡萄糖注射液中冰片和麝香酮含量

目的建立醒脑静葡萄糖注射液中冰片和麝香酮的含量测定方法。方法使用气相色谱仪，采用DM-17毛细管色谱柱（50％苯基和50％甲基聚硅氧烷），0.32mm×30m，液膜厚度为0.25μm；柱温70℃，保持2min，以10℃·min^-1的速度升温至200℃，保持10min。以氮气为载气，流速为2ml·min^-1；FID检测器。结果冰片，麝香酮线性关系良好，相关系数均大于0.999；平均回收率分别为97.2％、97.0％；检出限分别为0.25、0.16mg·L^-1。结论本方法简便，灵敏度高，重复性好，结果准确，适合于醒脑静葡萄糖注射液中冰片和麝香酮的含量测定。

Simultaneous determination of muscone and borneol of xingnaojing glucose injection by GC

Aim To establish a GC method for the simultaneous determination of muscone and borneol of xingnaojing glucose injection. Methods DM-17 capillary column （0.32 mm×30 m ×0.25 μm,coated with 50% phenyl and 50% methyl-polysiloxan） was adopted and the carrier gas was nitrogen with 2 ml·min^-1 of flow rate. The initial column temperature was kept at 70℃ for 2 min, then raised to 200℃ and kept for 10 rain at the rate of 10℃·min^-1. The detector was FID. Results The correlation coefficient of muscone and borneol were all higher than 0.999. The average recovery were 97.2% ,97.0% and the limit of detection were 0.25,0.16 mg·L^-1, re- spectively. Conclusion This method is sensitive, accurate, repeatable and easy to operate for the simultaneous determination of muscone and borneol of xingnaojing glucose injection.