不同产地九节木根HPLC指纹图谱研究＊

目的 建立九节木根的HPLC指纹图谱,综合比较不同产地的九节木根质量,为九节木根药材质量控制提供参考。方法 采用Kromasil C18 色谱柱（4.6 mm×250 mm,5 μm）;以乙腈（A）-0.4%磷酸溶液（B）为流动相,梯度洗脱;检测波长为232 nm;流速为1 mL·min^-1;进样量为10 μL。利用“中药色谱指纹图谱相似度评价系统（2004A版）”计算相似度,SPSS21软件进行聚类分析和主成分分析,比较不同产地九节木根样品的指纹图谱。结果 建立九节木根的HPLC指纹图谱,确定了29个共有峰。10批九节木根指纹图谱与对照图谱的相似度为0.922-0.996,10批次样品聚为3类。结论 该研究建立的九节木根指纹图谱具有较高的重现性、稳定性及专属性,为九节木药材的质量控制和评价的依据。

Study on the HPLC Fingerprint of Psychotria rubra Root from Different Regions

Objective To establish the HPLC fingerprint of root from Psychotria rubra, and comprehensively compare the quality of Psychotria rubra root from different producing areas, so as to provide reference for the quality control of Psychotria rubra root.Methods The chromatographic separation was performed on a Kromasil C18 column (4.6 mm×250 mm, 5 um). The mobile phase was acetonitrile (A) -0.4% phosphoric acid (B) with gradient elution. The detection wavelength was 232 nm, and the flow rate was 1.0 mL·min^-1, and injection volume was 10 uL. The fingerprint was compared for similarity by using "Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2004 A)". Cluster analysis and principal component analysis were adopted in SPSS21, and the HPLC chromatograms from Different Regions of Psychotria rubra root were compared.Results The HPLC fingerprint of Psychotria rubra root was established and 29 common peaks were designated. The similarities of 10 batchdes of samples were from 0.922 to 0.996. The 10 batches of samples were clustered into 3 categories.Conclusion The fingerprint of Psychotria rubra root established in this study has high reproducibility, stability and specificity, which is the basis for the quality control and evaluation of Psychotria rubra.