| 小鼠腹腔手术睾丸注射抑制模型的建立 |
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| 引用本文： | 邹高焕,许孟飞,唐雅君,江孙颖,郑舒敏,杨宇豪,魏雅岚,佘振宇.小鼠腹腔手术睾丸注射抑制模型的建立[J].福建医科大学学报,2021,55(5):382-385. |
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| 作者姓名： | 邹高焕 许孟飞 唐雅君 江孙颖 郑舒敏 杨宇豪 魏雅岚 佘振宇 |
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| 作者单位： | 福建医科大学基础医学院,福建医科大学基础医学院,福建医科大学基础医学院,福建医科大学基础医学院,福建医科大学基础医学院,福建医科大学基础医学院,福建省妇幼保健院,福建医科大学基础医学院 |
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| 基金项目： | 国家自然科学基金(82001608)；福建省自然科学基金青年创新项目(2019J05071)；福建医科大学高层次人才科研启动经费计划项目(XRCZX2017025)。 |
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| 摘 要： | 目的 建立小鼠睾丸注射模型,获得一种蛋白抑制的简便易行、高效且存活率高的方法,为研究小鼠精子发生的相关实验提供借鉴.方法 将50只小鼠随机分为对照组和实验抑制组.对照组注射10μL 0.05％台盼蓝或磷酸盐缓冲液,实验抑制组注射10μL 0.075％Dimethylenastron抑制剂.通过腹腔手术暴露小鼠睾丸,睾丸...
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| 关 键 词： | 腹腔手术 小鼠睾丸 抑制模型 精子发生 |
| 收稿时间： | 2021/1/18 0:00:00 |
| 修稿时间： | 2021/11/22 0:00:00 |
Establishment of testicular injection inhibition model in mice using abdominal surgery |
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| ZOU Gaohuan,XU Mengfei,TANG Yajun,JIANG Sunying,ZHENG Shumin,YANG Yuhao,WEI Yalan and SHE Zhenyu.Establishment of testicular injection inhibition model in mice using abdominal surgery[J].Journal of Fujian Medical University,2021,55(5):382-385. |
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| Authors: | ZOU Gaohuan XU Mengfei TANG Yajun JIANG Sunying ZHENG Shumin YANG Yuhao WEI Yalan and SHE Zhenyu |
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| Institution: | Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University,Medical Research Center,Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University,Fuzhou,Fujian,Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University |
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| Abstract: | AABSTRACT: Objective A mouse testicular injection model was established to obtain a simple, efficient and high survival rate method of protein inhibition, which can be used as a reference for related experiments and studies of spermatogenesis in mice. Methods The testes of mice were exposed by abdominal operation. Protein inhibitors were injected into mouse testes. The wound was sutured intermittently. Results Histological results showed that the chromosomes of spermatocytes in seminiferous tubules of the inhibition group were misaligned. The spermatocytes were arrested at metaphase and could not divide normally. This model can inhibit the proteins in the testes of mice, and the survival rates of mice were high. Discussions The commonly used method of testicular inhibition in mice is direct injection through the scrotum, however, the time window for operation is limited. Testicular injection through abdominal surgery in mice can achieve testicular inhibition in the 3-week-old mice with high success rate and good effects on protein inhibition. This method is suitable for the studies of male meiosis in spermatocytes.
KEY WORDS: abdominal surgery; mouse testis; inhibition model; spermatogenesis |
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| Keywords: | KEY WORDS: abdominal surgery mouse testis inhibition model spermatogenesis |
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