Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes |
| |
| Authors: | A. I. Khankoeva A. S. Dukhanin P. A. Galenko-Yaroshevskii |
| |
| Affiliation: | (1) Department of Pharmacology, Kuban Medical Academy, Krasnodar;(2) Department of Molecular Pharmacology with a Radiobiology Course, Russian State Medical University, Moscow |
| |
| Abstract: | Transmembrane potentials on the plasma (Δφ4) and mitochondrial (Δφm) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were −93±4 and −196±11 mV, respectively. Sufan significantly decreased the reduction of Δφm induced by chemical hypoxia. The effects of antiarrhythmic drugs on changes in Δφp were studied using the fluorescent probe dis-C3-(5). Lidocaine, novocainamide, richlocaine, and leocaine blocked depolarization of the myocyte plasma membrane induced by electrical stimulation and did not affect the Δφm. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 126, No. 11, pp. 594–597, November, 1998 |
| |
| Keywords: | transmembrane potential antiarrhythmic drugs cardiomyocytes |
| 本文献已被 SpringerLink 等数据库收录！ |
|
