HHV-6 cell receptor CD46 expression on various cell subsets of six blood and graft sources: A prospective series |
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| Authors: | Patrice Chevallier Nelly Robillard Marina Illiaquer Julie Esbelin Mohamad Mohty Celine Bodin-Bressollette Thierry Guillaume Veronique Stocco Fabienne Auffray Sophie Derenne Lucie Planche Marie-Christine Bene Berthe-Marie Imbert-Marcille |
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| Affiliation: | 1. Service d’Hématologie Clinique, CHU Hôtel Dieu, Université de Nantes, Centre d’Investigation Clinique en Cancerologie (CI2C) and INSERM U892, Nantes, France;2. Service d’Hematologie/Biologie, CHU de Nantes, France;3. Service de Virologie, CHU de Nantes, Nantes, France;4. UPRES-EA4271, PRES L’UNAM, Nantes University, France;5. Service de gynecologie/obstetrique, CHU de Nantes, Nantes, France;6. Unité d’ingénierie cellulaire, EFS Pays de la Loire, Nantes, France;7. Cellule de Promotion à la Recherche Clinique, CHU de Nantes, Nantes, France;1. Department of Clinical Medicine, University of Bologna, Bologna, Italy;2. Operative Unit of Microbiology and Virology, Sant’Orsola-Malpighi Hospital, Bologna, Italy;3. Scientific Affairs, Abbott Diagnostici, Roma, Italy;1. Pediatric Clinic 1, Department of Pathophysiology and Transplantation, Università degli Studi di Milano, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy;2. The Pediatric Infectious Disease Unit, Soroka University Medical Center, Beer-Sheva, Israel;3. The Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel;4. Molecular Virology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy;1. Department of Medical Microbiology, Malmö University Hospital, Lund University, Jan Waldenströms gata 59, 20502 Malmö, Sweden;2. Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zalo?ka 4, 1000 Ljubljana, Slovenia;3. National Institute of Public Health, Trubarjeva 2, 1000 Ljubljana, Slovenia;4. Department of Laboratory Medicine, Karolinska Institute and Karolinska Hospital, Stockholm, Sweden;5. Department of Medical Epidemiology and Biostatistics, Karolinska Institute Nobels väg 12, 171 77 Stockholm, Sweden;1. Service de Gastroentérologie et Hépatologie, Fédération des Spécialités Digestives, Hôpital Edouard Herriot, Lyon, France;2. Service de Pharmacologie, Hôpital Edouard Herriot, Lyon, France;3. Clinique Universitaire de Néphrologie, CHU A Michallon, Grenoble, France;4. Service de Néphrologie, Transplantation et Immunologie Clinique, Hôpital Edouard Herriot, Lyon, France;5. Clinique Universitaire d’Hépatogastroentérologie et INSERM U823, CHU A Michallon, Grenoble, France;1. Unit of Infectious Diseases, Instituto de Investigación Hospital 12 de Octubre (i+12), University Hospital 12 de Octubre, Universidad Complutense, Madrid, Spain;2. Department of Nephrology, Instituto de Investigación Hospital 12 de Octubre (i+12), University Hospital 12 de Octubre, Universidad Complutense, Madrid, Spain;3. Department of Microbiology, Instituto de Investigación Hospital 12 de Octubre (i+12), University Hospital 12 de Octubre, Madrid, Spain;4. Department of General Surgery, Alimentary Tract and Abdominal Organ Transplantation, Instituto de Investigación Hospital 12 de Octubre (i+12), University Hospital 12 de Octubre, Madrid, Spain |
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| Abstract: | BackgroundCord Blood (CB) are increasingly used as an alternative stem cells source in adults for allogeneic Stem Cell Transplantation (allo-SCT). The risk of human herpesvirus (HHV-6) reactivation is significantly higher after CB transplant vs unrelated peripheral blood stem cells (PBSC) allo-SCT. Higher HHV-6 cell receptor CD46 expression on progenitor cells in CB may explain this difference.ObjectivesTo prospectively compare the HHV-6 cell receptor CD46 expression on various cell subsets of three freshly harvested blood sources on one hand and of three graft sources on the other hand.Study design52 samples were used for the purpose of this study. They were issued from peripheral blood (PB, n = 10), G-CSF mobilised PB (GCSF-PB, n = 10), cord blood (CB, n = 10), unmanipulated bone marrow (uBM, n = 5), leukapheresis product (LP, n = 10) and thawed CB graft (n = 7). CD46 expression was assessed by FACS analysis on total lymphocytes, monocytes, NK cells, T and B cells subsets, plasmacytoid (pDCs) dendritic cells and stem cells.ResultsAs all cell subsets were found CD46 positive, CD46 mean fluorescence intensity (MFI) was then considered for comparison between the three blood sources and the three graft sources. The most impressive result observed was that HHV-6 cell receptor CD46 expression was significantly reduced in almost all cell components of thawed CB graft compared to other graft sources.ConclusionsThis original study shows strong differences in term of quantitative CD46 expression between several blood and grafts samples. Our results suggest that other factors than the qualitative CD46 expression play a role in the higher HHV-6 reactivation observed after CB transplant in adults. |
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