| 含抑癌基因p16转移载体的构建 |
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| 引用本文： | 马春红,孙汶生,张利宁,曹英林,宋静,刘素侠. 含抑癌基因p16转移载体的构建[J]. 山东大学学报(医学版), 2000, 38(3): 243-244 |
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| 作者姓名： | 马春红 孙汶生 张利宁 曹英林 宋静 刘素侠 |
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| 作者单位： | 山东医科大学微生物学教研室 |
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| 基金项目： | 卫生部科研基金资助课题(96-2158) |
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| 摘 要： | 目的为获得高表达有活性的p16蛋白，构建含p16cDNA的重组转移载体。方法EcoRI-xhoI双酶切克隆质粒pBLUESCRIPT-p16，低融点琼脂糖回收0．8kb的p16cDNA片段，插入质粒pSXIVVI
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| 关 键 词： | 基因，抑制，肿瘤；基因，p16；转移载体；杆状病毒科 |
| 文章编号： | 1000-0496(2000)03-0243-02 |
| 修稿时间： | 1999-05-08 |
CONSTRUCTTION OF RECOMBINED TRANSFER VECTOR CARRYING p16 cDNA |
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| MA Chun-hong. CONSTRUCTTION OF RECOMBINED TRANSFER VECTOR CARRYING p16 cDNA[J]. Journal of Shandong University:Health Sciences, 2000, 38(3): 243-244 |
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| Authors: | MA Chun-hong |
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| Abstract: | Objective:To get plenty of active p16 protein,and build a recombined transfer vector with p16 cDNA. Methods:p16 cDNA carried by cloning vector pBLUESCRIPT p16 was cut down with EcoRI and xhoI,then collected and purified by low melting agarose.The 800bp long p16 cDNA was inserted into transfer vector carrying p16 cDNA(pX 3 p16)was selected and identified by in situ hybridization and electrophoresis. Results: 800bp of p16 cDNA purfied from low melting agarose war assayed by electrophoresis.8 positive clones were obtanined after in situ hybridization with p16 cDNA probe,and the transfer vector carrying with p16 cDNA(pX 3 p16)was identified by digestion of EcoR I and xhoI. Conclusion:pX 3 p16 was a recombiant of p16 cDNA and pSXIVVI X 3. |
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| Keywords: | Genes suppressor tumor Genes p16 Transfer vector Baculoviridac |
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