HPLC—MS法测定克拉霉素浓度

目的建立快速、简便的HPLC—MS法测定人血浆中克拉霉素浓度的方法。方法以罗红霉素为内标，采用Shim—packODSC18柱（250mm×2．0mm5m）甲醇-水溶液（含0．1％甲酸）（60：40v／v）为流动相，流速0．2mL／min，柱温30℃。采用质谱电喷雾离子源正源（ESI＋）将样品离子化，选择性离子监涮（SIM）准分子离子峰。结果克拉霉素在0．025～6．4μg／ml范围内线性关系良好（r=0．999），最低定量限为0．025μg／ml，提取回收率均大干85％，批内与批间RSD均小于15％。结论该方法简单，快速、灵敏度高、专属性强．可较好应用于临床克拉霉素血浆浓度检测。

Application of determining the concentration of clarithromycin hydrochloride in human plasma by HPLC-MS

Objective A rapid and simple high performance liquid chromatography method with UV detection was established for determining the concentration of clarithromycin in human plasma. Methods Diazepam was used as internal standard. Solutes were separated on a Shim-pack ODS C18 column（ 150mm × 4.6mm 5m） with a mo- bile phase of acetonitrile-H2O （60 ： 40）, The flow rate wasl. 0 ml/min and column temperature was 30℃ . clarithromycin was ionized in the eletrospray ionization （ESI} with positive source of the mass spectrometer and detected in selected ionization monitor （SIM} mode. Results The Linearity was good in the linear range of 0. 025 64μg/ml; the lower limit of quantization was0. 025μg/ml,The precise of intra- and inter-day were less than 15 % and recovery was more than 85 %. Conclusion The method was rapid, sensitive and reliability, which can use to detect the plasma concentration of clarithromycin in clinic.