支气管哮喘患者粒—基噬细胞集落刺激因子mRNA表达的研究

目的 探讨利用无创伤性方法寻找较为特异性地反映哮喘患气道炎症的客观指标。方法 通过研究哮喘患气道粘膜细胞粒－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）ｍＲＮＡ表达，并以此为参照，分析哮喘患诱导痰细胞ＧＭ－ＣＳＦ ｍＲＮＡ变化，利用免疫组化结合逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术，分析哮喘组（１０例）气道粘膜细胞ＧＭ－ＣＳＦ ｍＲＮＡ的表达；用３％高渗盐水诱导痰液，利用ＲＴ－ＰＣＲ技术分析哮喘组（

Expressionof GM-CSF mRNA in asthmatic patients

OBJECTIVE: To explore non-invasive and specific markers for airway inflammation. METHODS: RT-PCR methods were used to measure GM-CSF mRNA expression in airway mucosa and induced sputum from asthmatic patients. The correlation was analyzed between expression of GM-CSF mRNA and eosinophils or other inflammatory cells. RESULTS: (1) The numbers of eosinophils and EG2 positive cells were (17.9 +/- 7.0) x 10(2) per mm2, and (8.9 +/- 3.0) x 10(2) per mm2 in the asthmatic group, significantly higher than those in the non-asthmatic control which were (1.9 +/- 1.0) x 10(2) per mm2, and (0.8 +/- 0.2) x 10(2) per mm2, respectively (P < 0.05). (2) The expression of GM-CSF mRNA was (1.4 +/- 0.6) in the asthmatic group, significantly higher than that in the control group (0.3 +/- 0.3) (P < 0.05), and GM-CSF mRNA expression was correlated with the numbers of EG2 positive cell (r = 0.73 and P < 0.05). (3) The percentage of eosinophils in induced sputum from asthmatic patients was (0.334 +/- 0.067), which was significantly higher than that from chronic bronchitis and control (0.021 +/- 0.004), (0.008 +/- 0.003) (P < 0.05). (4) The expression of GM-CSF mRNA in asthmatics was (0.320 +/- 0.054), significantly higher than that in chronic bronchitis and the control group (0.188 +/- 0.024 and 0.058 +/- 0.028) (P < 0.05). The expression of GM-CSF mRNA in patients with chronic bronchitis was also significantly higher than that in the control group (P < 0.05). CONCLUSIONS: The expression of GM-CSF mRNA in induced sputum may be a relatively specific marker for airway inflammation.