| 藏药秦艽、麻花艽中落干酸和龙胆苦甙的HPLC法含量测定 |
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| 引用本文： | 林鹏程,冶兆辉,卢永昌,赵素琴,胡凤祖,纪兰菊.藏药秦艽、麻花艽中落干酸和龙胆苦甙的HPLC法含量测定[J].中药材,2004,27(11):819-821. |
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| 作者姓名： | 林鹏程 冶兆辉 卢永昌 赵素琴 胡凤祖 纪兰菊 |
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| 作者单位： | 1. 青海民族学院化学系,青海西宁,810007
2. 中国科学院西北高原生物研究所,青海西宁,810001 |
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| 摘 要： | 应用反相高效液相色谱法同时测定藏药秦艽、麻花艽中落干酸、龙胆苦甙含量.并比较了加热回流提取及超声提取两种方法对分析结果的影响.还测定了两种藏药全草及根、茎、叶、花等不同部位两种成分的含量.
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| 关 键 词： | 反相高效液相色谱 落干酸 龙胆苦甙 秦艽 麻花艽 |
| 修稿时间： | 2004年3月24日 |
Content Determination of Loganic Acid and Gentiopicroside in Tebitan Herbal Medicines Gentiana macrophylla and G.straminea by HPLC |
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| Lin Pengcheng ,Ye Zhaohui ,Lu Yongchan ,Zhao Suqin ,Hu Fengzu ,Ji Lanju.Content Determination of Loganic Acid and Gentiopicroside in Tebitan Herbal Medicines Gentiana macrophylla and G.straminea by HPLC[J].Jorunal of Chinese Medicinal Materials,2004,27(11):819-821. |
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| Authors: | Lin Pengcheng Ye Zhaohui Lu Yongchan Zhao Suqin Hu Fengzu Ji Lanju |
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| Institution: | Qinghai Nationalities Institutes, Xining. |
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| Abstract: | A quantitative method to determine loganic acid and gentiopicroside in Gentiana macrophylla Pall. and Gentiana straminea Maxim. by RP-HPLC was established in this paper. The two compounds were base-isolated on the coloumn of Zorbax SB-C18 (250 mm x 4.6 mm i.d., 5 microm) which eluted with MeOH and H2O (0.04% H3PO4). The methol ratio increased from 18% to 28% in 15 min with detective wave at 254 nm and coloumn temperature at 30 degree C, flow rate at 1 ml/min. A wide range of linearity and the good linear relationships were achieved on above condition. The contents of the two compounds in different parts of the two plants were also determined and compared in this paper. |
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| Keywords: | HPLC Loganic acid Gentiopicroside Gentiana macrophylla Gentiana straminea |
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