质粒谱分析用于痢疾流行病学调查的初步探讨

本文报道了1985年从北京市的海淀区和密云县以及浙江省湖州地区分离到的134株痢疾杆菌进行质粒谱分析的结果,发现在每一菌群的痢疾杆菌内均有许多不同的质粒谱,推测在当地痢疾的流行可能是由许多在遗传学上不同的痢疾杆菌引起的;而且质粒谱的数量一般超过耐药谱数,说明前者能比后者鉴别更多的菌株。同时又发现,在同一地区内在爆发流行的短时期(一个月)内分离到的痢疾杆菌中,尽管耐药谱不同,部分菌株可呈现为相同的质粒谱。所以,质粒谱分析有可能做为痢疾流行病学调查工具之一,用于鉴别菌株、鉴定传染源、追踪耐药性质粒或评价防治效果。

A Preliminary Study on the Usefulness of plasmid Profile Analysis for the Epidemiological Investigation of Shigellosis

This paper gives experimental results of plasmid profile analysis of 134 strains of Shigella isolated from 3 different areas (Hai Dian District and Mi Yun County, Beijing and Hu Zhou City, Zhe Jiang Province)
The results showed that many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella were responsible for dysentry epidemics in the three areas; and the number of different plasmid profiles was more than the number of different antimicrobial susceptibility patterns, indicating that plasmid profiles might distinguish more strains than did the drug resistance patterns.
On the other hand, some of Shigella isolates recovered within a 1-month period from an outbreak, in the same area showed a same plasmidprofile, in spite of their different drug resistance patterns.
The above results suggested that plasmid profile analysis might be useful as an epidemiological tool in investigating outbreaks of Shigellosis for differentiation of strains identifying a source of infection, tracing R plansmids, or evaluating the efficacy of control measures.