新的高血压相关基因- Slc7a8的实验研究

目的:探讨高血压致病相关基因。方法:提取13周龄SHR和WKY的2、3级肠系膜动脉和肾脏总RNA,利用含10000个基因大鼠表达谱芯片检测2组2、3级肠系膜动脉和肾脏组织基因表达水平不同的基因,并用定量RT-PCR的方法排除假阳性候选基因。结果:芯片发现19个基因在SHR中上调,其中涉及分子伴侣、离子通道和小分子转运、生长因子、细胞信号转导的蛋白和核转录因子、脂蛋白等基因。用定量RT-PCR验证示Slc7a8基因的表达水平在SHR组中比WKY组上调9.3倍。结论:Slc7a8可能与高血压的形成有关,深入研究Slc7a8基因及其功能将为进一步了解高血压病的分子机制提供新的思路和线索。

Experimental study of a new hypertension -related gene Slc7a8

AIM: To investigate the disease related genes in SHR. METHODS: The total RNA samples were obtained from second-order mesenteric arteries and kidney of SHR and WKY. Microarray containing over 10 000 genes was used to determine the level of mRNA expression in two groups. The genes were identified using real time quantitative RT- PCR.RESULTS: 19 down-regulated genes were determined by microarray, which were classified as chaperones, transport, growth factors, signal transduction, nuclear factor and lipoprotein. The result was confirmed by the method of real time quantitative RT- PCR. It was found that the Slc7a8 gene was up-regulated 9.3 fold in SHR. CONCLUSION: Slc7a8 gene may relate to hypertension. Further study on the Slc7a8 gene and its function would help us wholly understand the mechanism of hypertension and provide new clue to hypertension causes.