Protection in mice immunized with a heterologous prime-boost regime using DNA and recombinant pseudorabies expressing TgSAG1 against Toxoplasma gondii challenge |
| |
Authors: | Limin Shang Quan Liu Wensen Liu Jingtao Men Shengyan Gao Li Jiang Ze Wang Yujia Zhai Hongtao Jin Hai Lian Chen Chen Zhiping Xia Ziguo Yuan Xing-Quan Zhu |
| |
Affiliation: | 1. Laboratory of Parasitology, Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, Jilin Province, China;2. Department of Parasitology, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, Guangdong Province, China |
| |
Abstract: | An effective vaccine of animals can block transmission of Toxoplasma gondii to humans. In this study, mice have been protected against lethal T. gondii challenge by a prime-boost vaccination strategy using DNA vaccine pVAX/TgSAG1 and recombinant pseudorabies virus rPRV/TgSAG1, both expressing the major immunodominant surface antigen of T. gondii (TgSAG1). High levels of splenocyte proliferative responses and significant levels of IFN-γ resulted, with strong cytotoxic T lymphocyte (CTL) responses in vitro. After lethal challenge, prime-boost vaccinated mice showed an increased survival time (15.4 ± 5.0 days) and a 40% survival rate compared with controls who all died within 11 days of challenge. Results of the present study indicated that this novel immunization strategy is useful in enhancing immune protection in mice against lethal T. gondii infection, which would provide foundation for the development of effective vaccines against T. gondii. |
| |
Keywords: | Toxoplasma gondii TgSAG1 Pseudorabies virus DNA vaccine Prime-boost regime Mice |
本文献已被 ScienceDirect 等数据库收录! |
|