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Synthetic peptides coupled to the surface of liposomes effectively induce SARS coronavirus-specific cytotoxic T lymphocytes and viral clearance in HLA-A*0201 transgenic mice |
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| Authors: | Satoshi Ohno Shunsuke Kohyama Maiko Taneichi Osamu Moriya Hidenori Hayashi Hiroshi Oda Masahito Mori Akiharu Kobayashi Toshitaka Akatsuka Tetsuya Uchida Masanori Matsui |
| Affiliation: | 1. Department of Microbiology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495, Japan;2. Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado-city, Saitama 350-0295, Japan;3. Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, Musashimurayama-city, Tokyo 208-0011, Japan;4. Drug Delivery System Development Division, Nippon Oil and Fat Corporation, Tokyo 150-6019, Japan |
| Abstract: | We investigated whether the surface-linked liposomal peptide was applicable to a vaccine based on cytotoxic T lymphocytes (CTLs) against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV). We first identified four HLA-A*0201-restricted CTL epitopes derived from SARS-CoV using HLA-A*0201 transgenic mice and recombinant adenovirus expressing predicted epitopes. These peptides were coupled to the surface of liposomes, and inoculated into mice. Two of the liposomal peptides were effective for peptide-specific CTL induction, and one of them was efficient for the clearance of vaccinia virus expressing epitopes of SARS-CoV, suggesting that the surface-linked liposomal peptide might offer an effective CTL-based vaccine against SARS. |
| Keywords: | SARS coronavirus Liposomal peptides Cytotoxic T lymphocytes |
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