Immunological assessment of plant-derived avian flu H5/HA1 variants |
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Authors: | S Spitsin V Andrianov N Pogrebnyak Y Smirnov N Borisjuk C Portocarrero V Veguilla H Koprowski M Golovkin |
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Institution: | 1. Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107-6799, United States;2. Centers for Disease Control and Prevention, Atlanta, GA 30333, United States |
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Abstract: | Polypeptide variants of the HA1 antigenic domain of the H5N1 avian influenza virus hemagglutinin (HA) molecule were produced in plants using transient and stable expression systems and fused with His/c-myc tags or with mouse or human Fc antibody fragments. The resulting peptides were purified and used for intramuscular immunization of mice. While the recombinant HA1 variants induced a significant serum humoral immune response in the mice, none of the HA1 preparations induced virus-neutralizing antibodies. Fusion with the Fc fragment improved overall yield of the constructs and allowed purification requiring only a single step, but led to no detectable fusion-related enhancement of immunogenicity or quality of immune response. |
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Keywords: | HA hemagglutinin pHA plant-derived HA1 antigen pHA1-mFc or pHA1-hFc pHA1 fused with mouse or human heavy chain IgG Fc fragment |
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