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Prime–boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI |
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| Authors: | M Steensels M Bublot S Van Borm J De Vriese B Lambrecht A Richard-Mazet S Chanavat-Bizzini M Duboeuf F-X Le Gros T van den Berg |
| Institution: | 1. Avian Virology & Immunology Unit, Veterinary & Agrochemical Research Centre (VAR), 99 Groeselenberg, B1180 Brussels, Belgium;2. Merial S.A.S., R&D, 254 rue M. Mérieux, 69007 Lyon, France |
| Abstract: | The efficacy of different vaccination schedules was evaluated in 17-day-old Pekin ducks using an experimental inactivated whole virus vaccine based on the H5N9 A/chicken/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing a synthetic HA gene from an Asian H5N1 isolate (A/chicken/Indonesia/7/2003). Full protection against clinical signs and shedding was induced by the different vaccination schemes. However, the broadest antibody response and the lowest antibody increase after challenge were observed in the group of ducks whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, suggesting that this prime-boost strategy induced optimal immunity against H5N1 and minimal viral replication after challenge in ducks. In addition, this prime-boost vaccination scheme was shown to be immunogenic in 1-day-old ducklings. |
| Keywords: | AI avian influenza AsHP H5N1 Asian highly pathogenic H5N1 BPL beta-propiolactone BSL3 biosecurity level 3 dpch days post challenge dpV1 days post first vaccination dpV2 days post second vaccination EID50 50% egg infectious dose H5N9-It A/chicken/Italy/22A/98 H5N9 whole virus inactivated vaccine HA hemagglutinin HI hemagglutination inhibition HPAI highly pathogenic avian influenza LPAI low pathogenic avian influenza PBS phosphate buffered saline pch post challenge (RT)2-PCR real-time RT-PCR SPF specific pathogen free Tr room temperature TCID50 50% tissue culture infectious dose vFP-H5 fowlpox recombinant containing the synthetic A/chicken/Indonesia/7/2003 H5N1 HA gene modified at the cleavage site vFP89 fowlpox recombinant containing the H5 of A/turkey/Ireland/83 (HP H5N8) |
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