As2O3磁性纳米微球磁感应加热治疗宫颈癌的研究

目的探讨As2O3磁性纳米微球磁感应加热治疗宫颈癌的作用和机理。方法用培养的SiHa细胞建立裸鼠人宫颈癌移植瘤模型,将研制的As2O3纳米微球、磁性纳米微球、As2O3磁性纳米微球等注入肿瘤细胞接种处,加高频交变磁场磁感应加热治疗3次后,d 45实验结束,分析比较3个实验组较对照组的延迟出瘤时间、质量抑制率、肝功能和肾功能情况及肿瘤、瘤周和内脏组织学的改变。同时观察磁流体热疗的升温和恒温效果。结果在高频交变磁场下,As2O3磁性纳米微球组肉眼未见肿瘤生长,该组的肿瘤质量抑制率为99.93%,明显高于As2O3纳米微球组(85.20%,P<0.01)及磁性纳米微球组(87.72%,P<0.01)。各实验组裸鼠血清AST、ALT、Bun及Cr水平与对照组相比无显著性差异(P>0.05),瘤旁和内脏组织未见明显损伤。结论在高频交变磁场作用下,As2O3纳米微球、磁性纳米微球、As2O3磁性纳米微球治疗裸鼠宫颈癌均有效,以As2O3磁性纳米微球效果最佳。本研究所采用的As2O3磁性纳米微球联合磁流体热疗技术具有双重抗癌功能,为宫颈癌和其他实体瘤的治疗提供了一条新途径。 

Treatment of Cervical Carcinoma with As2O3 Nanometer Microballoon Magnetism Induced Heating

OBJECTIVE To explore into the effect and mechanism of using As_2O_3 nanometer microballoon magnetism induced heating in the treatment of cervical carcinoma. METHOD Athymic mouse transplantation tumor models of human cervical carcinoma was made using SiHa cells.As_2O_3 nanometer microballoons,magnetic nanometer microballoons and As_2O_3 magnetic nanometer microballoons were then infused into the inoculation location of the tumor cells,which were then heated for 3 times with high-frequency alternating magnetic field.The experiment lasted for 45 days. RESULT Under the high-frequency alternating magnetic field,no tumor growth was detected in the As_2O_3 magnetic nanometer microballoon group,the tumor growth inhibiting rate being 99.93%,markedly higher than the As_2O_3 nanometer microballoon group(85.20%,P<0.01) and the magnetic nanometer microballoon group(87.72%,P<0.01).No marked differences were detected between the experimental groups and the control group in serum AST,ALT,Bun and Cr(P>0.05).No marked lesion was found in the tissue around the tumors and internal organs.CONCLUSION Under the high-frequency alternating magnetic field,As_2O_3 nanometer microballoons, magnetic nanometer microballoons and As_2O_3 magnetic nanometer microballoons are all effective in treating cervical carcinoma,especially As_2O_3 magnetic nanometer microballoons.As_2O_3 magnetic nanometer microballoons used in combination with fluid thermotherapy has dual anti-cancer effect.