快速眼动睡眠剥夺对抑郁模型大鼠纹状体5-HTDA及腺苷的影响

目的：通过对抑郁模型大鼠的快速眼动睡眠剥夺，观察大鼠强迫游泳不动时间及大鼠纹状体5-羟色胺、多巴胺和腺苷水平的变化，了解睡眠剥夺快速改善抑郁情绪的可能机制。方法将30只成年健康雄性大鼠分为正常对照组（A组）、抑郁模型组（B组）、抑郁模型＋睡眠剥夺组（C组），在建立慢性轻度不可预见性应激的抑郁模型后，采用小平台水环境法对大鼠进行72 h快速眼动睡眠剥夺，以强迫游泳实验检测大鼠的不动时间，采用高效液相色谱-荧光检测法测定3组大鼠纹状体5-羟色胺、多巴胺水平，采用高效液相色谱-紫外检测法测定3组大鼠纹状体腺苷水平。结果21 d慢性轻度不可预见性应激后，大鼠的强迫游泳不动时间显著延长（ P＜0．05），快眼动睡眠剥夺后大鼠强迫游泳不动时间显著缩短（P＜0．01）。A组、C组大鼠纹状体5-羟色胺及腺苷水平均显著高于B组（ P＜0．05或0．01），C组大鼠纹状体多巴胺水平显著高于A组、B组（P＜0．01）。结论快速眼动睡眠剥夺可以逆转大鼠的抑郁样行为，并提高大鼠纹状体5-羟色胺、多巴胺水平，腺苷可能参与了睡眠剥夺的抗抑郁过程。

Effects of REM sleep deprivation on 5-HT,DA and adenosine in striatum of depression-model rats

Objective To observe rats’ motionless time during forced swimming and changes of 5-HT ,DA and adenosine in striatum via depriving REM sleep of depression-model rats and to find out possible mecha-nism of REM sleep deprivation improving emotional emotion .Methods Thirty healthy male adult rats were divided into normal control group （group A） ,depression-model （group B） and depression-model with sleep deprivation （group C） ,72-hour REM sleep deprivations were carried out using “flower pot” tech-nique after establishing chronic mild unpredictable stress depression-model ,motionless times detected u-sing forced swimming ,striatum 5-HT and DA levels measured with high efficiency liquid chromatography-fluorescence detection and adenosine with high efficiency liquid chromatography-ultraviolet .Results Mo-tionless time during forced swimming prolonged notably after 21-day chronic mild unpredictable stresses , （P〈0 .05） and shortened after REM sleep deprivation （P〈0 .01） .Striatum 5-HT and adenosine levels were significantly higher in both group A and C than B （P〈0 .05 or 0 .01） ,so were the DA in group C than A and B （P〈0 .01） .Conclusion REM sleep deprivation could reverse the depression-like behaviors of rats and increase 5-HT and DA levels in striatum ,adenosine may take part in the antidepressant process of REM sleep deprivation .