雷公藤根化学成分的分离与鉴定

目的研究雷公藤(Tripterygium wilfordiiHook.f.)根的化学成分。方法采用硅胶柱色谱、Sephadex LH-20、开放ODS柱色谱、HPLC等各种色谱技术对雷公藤根的化学成分进行分离纯化;根据理化常数测定和光谱分析对分离得到的化合物进行结构鉴定。结果从雷公藤根乙醇提取物中分离得到7个化合物,分别鉴定为2α,3α,23-三羟基乌苏烷-12-烯-28-酸(2α,3α,23-trihydroxyurs-12-en-28-oic acid,1)、2α,3α,23-三羟基乌苏烷-12,20(30)-二烯-28-酸(2α,3α,23-trihydroxyurs-12,20(30)-dien-28-oic acid,2)、2α,3α,24-三羟基乌苏烷-12-烯-28-酸(2α,3α,24-trihydroxyurs-12-en-28-oic acid,3)、2α,3α,19α-三羟基乌苏烷-12-烯-28-酸(euscaphic acid,4)、熊果酸(urasolic acid,5)、齐墩果酸(oleanolic acid,6)、2S,2′S-aurantiamide acetate(7)。结论化合物1、2、3、7为首次从雷公藤属植物中分离得到。

Isolation and identification of chemical constituents of roots of Tripterygium wilfordii Hook. f.

Objective To study the chemical constituents from the roots of Tripterygium wilfordii Hook.f. . Methods The constituents were isolated by chromatographic methods such as silica gel, ODS column chroma tography, Sephadex LH-20 and HPLC. Physico-chemical properties and spectroscopic analysis were employed for the structural identification. Results Seven compounds were obtained and their structures were identified as 2α, 3α, 23-trihydroxyurs-12-en-28-oic acid (1), 2α, 3α, 23-trihydroxyurs-12, 20(30) -dien-28-oic acid (2), 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid (3), euscaphic acid (4), ursolic acid (5), oleanolic acid (6) and aurantiamide acetate (7). Conclusion Compounds 1, 2, 3 and 7 are obtained from this genus for the first time.