组织型纤溶酶原激活剂对人视网膜色素上皮细胞的抑制

目的：探讨组织型纤溶酶原激活剂（tissue-type plasminogen activator,tPA)对人视网膜色素上皮（retinal pigment epithelial,RPE)细胞的作用。方法：运用活细胞计数法和甲基噻咪基四唑（methyl thiazolyl tetrazolium,MTT)比色实验观察分析tPA对人RPE细胞增殖的抑制作用，应用流式细胞仪（flow eytometry,FCM)分析人RPE细胞周期。结果：0．1－3μg/ml tPA对人RPE细胞有抑增殖作用，其效应与药物浓度和作用时间呈正向依赖关系（P＜0．01）；5μg/ml tpA对人RPE细胞有致死毒性作用。RCM显示tPA作用后S期细胞增加9．8％（P＜0．05），G2M期减少6．6％（P＜0．05）。结论：tPA在一定时间和剂量范围内对人RPE细胞具有抑增殖作用，且不对细胞产生致死毒性；tPA主要延迟／阻滞人RPE细胞于S期。

Inhibition of Cultured Human Retinal Pigment Epithelial Cell by Tissue-type Plasminogen Activator

PURPOSE: To study the inhibition effects of human retinal pigment epithelial (RPE) cells by tissue-type plasminogen activator(tPA). METHODS: The effects of tPA on human RPE cells proliferation were studied by viable cell counting and methyl thiazolyl tetrazolium (MTT) colorimetric assay. Flow cytometry (FCM) analysis was used to examine RPE cell cycles. RESULTS: The proliferation of human RPE cells could be inhibited by 0.1-3 micrograms/ml tPA (P < 0.05). The effects of inhibition were depended on the drug density and the action time directly (P < 0.01). 5 micrograms/ml tPA was deadly-toxic to human RPE cells. FCM revealed that the cells in S phase increased 9.8% (P < 0.05) and cells in G2M phase decreased 6.6% (P < 0.05), compared to controls. CONCLUSION: tPA at a certain dose range and period could inhibit human RPE cells proliferation without deadly-toxicity. The human RPE cells growth could be interfered in S phase by tPA.