| 高效液相色谱法测定和胃散中橙皮苷的含量 |
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| 引用本文： | 崔永青,刘光斌,毛和平,隆旭红. 高效液相色谱法测定和胃散中橙皮苷的含量[J]. 航空航天医药, 2010, 21(4): 440-441 |
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| 作者姓名： | 崔永青 刘光斌 毛和平 隆旭红 |
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| 作者单位： | 1. 甘肃省酒泉钢铁公司医院,甘肃,嘉峪关,735100
2. 甘肃省嘉峪关市药品检验所,甘肃,嘉峪关,735100 |
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| 摘 要： | 目的：建立同时测定和胃散中橙皮苷含量的高效液相色谱法。方法：色谱柱：InertsilODS-SP（150mm×4.6mm,5μm）;流动相：乙腈-1%醋酸（28∶72）;流速：1.0mL/min;检测波长：283nm;柱温30℃。结果：橙皮苷在7.0～70.0μg/mL范围内质量浓度与峰面积均呈良好的线性关系;平均回收率为100.5%（RSD=0.82%,n=9）。结论：本法操作简便,准确度高,重现性好,可作为和胃散质量控制方法之一。
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| 关 键 词： | 和胃散 橙皮苷 HPLC |
Determination of Hesperidin in Hewei Powder by HPLC |
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| Affiliation: | CUI Yong -qing,LIU Guang-bin,MAO He -pin,et al (Jiugang Hospital of Gansu Provinse,Jiayuguan 735100 China) |
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| Abstract: | Objective：To develop a HPLC method for determining hesperidin in Hewei Powder. Methods：The analysis was performed on Inertsil ODS-SP （150 mm × 4. 6 mm,5 μm） with mixture of acetonitrile and 1% acetic acid as mobile phase. The detection wavelength was set at 283 nm and the column temperature was at 30 ℃. Results：The linear calibration curves were obtained in the concentration range of 7. 0 ～ 70. 0 μg /mL for hesperidin. The average recoveries of hesperidin was 100. 5% （RSD = 0. 82% ,n = 9）. Conclusions：The method is quick,simple,and reproducible,which can be used to control the quality of Hewei Powder. |
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| Keywords: | HPLC |
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