Role of Thrombin in Activation of Neurons in Rat Hippocampus |
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Authors: | E V Kiseleva T P Storozhevykh V G Pinelis E Gluza S M Strukova |
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Institution: | Biological Faculty, M. V. Lomonosov Moscow State University. |
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Abstract: | The effect of thrombin, an agonist of proteinase-activated receptor (PAR) family, was studied on cultured rat hippocampal neurons. Thrombin in a concentration range of 1 pM - 10 nM induced a transitory dose-dependent increase in intracellular free calcium concentration. Involvement of PAR1 in neural response to thrombin was corroborated in experiments with TFLLRN, a selective synthetic peptide agonist of these receptors. In a calcium-free medium and after treatment with cyclopiazonic acid (inhibitor of Ca(2+)-ATPase in the endoplasmic reticulum) activation of PAR not only mobilized Ca(2+) from intracellular stores, but also induced Ca(2+) entry into the cells. Thrombin decreased Ca(2+) signal triggered by activation of NMDA-subtype glutamate receptors. |
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Keywords: | thrombin PAR neurons hippocampus fluorescent microscopy intracellular calcium |
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