| Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials |
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| 引用本文： | 王光林,林卫,杨志明,裴福兴,刘雷. Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials[J]. 中华创伤杂志(英文版), 2003, 6(4): 209-212 |
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| 作者姓名： | 王光林 林卫 杨志明 裴福兴 刘雷 |
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| 作者单位： | [1]DepartmentofOrthopaedics,theWestChinaHospital,SichuanUniversity,Chengdu610041,China [2]DepartmentofObstetricsandGynecology,theWestChinaHospital,SichuanUniversity,Chengdu610041,China |
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| 基金项目： | This study was supported by the National Natural Science Foundation of China(No.30100190) |
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| 摘 要： | Objective: To study the adhesion, migration and three-dimentional growth of Schwann cells on PLA(polylactic acid) nonspinning fibre cloth and polyglycolic/polylactic acid (PLGA) fibres. Methods: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen, polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal microscope. Results : Schwann cell/ECM solution was compounded with PLA nonspinning fibre cloth. With formation of gel, most Schwann cells resided in the fibre net holes, and adhered to the fibres to form a multiplayerarranged Schwann cell column like Biingner band.Schwann cells could adhere to PLGA fibres and grew and migrated along the fibres. ECM gel could significantly increase the adhering and migrating cell number. Conclusions: ECM gel can facilitate the adhesion,growth and migration of Schwann cells on the seteroframe.It is a good integrating material for tissue engineering bioartificial nerve.
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| 关 键 词： | 许旺氏细胞 生物材料 组织相容性 细胞粘附作用 细胞培养 |
Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials |
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| WANG Guang-lin,LIN Wei,YANG Zhi-ming,PEI Fu-xing and LIU Lei. Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials[J]. Chinese journal of traumatology, 2003, 6(4): 209-212 |
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| Authors: | WANG Guang-lin LIN Wei YANG Zhi-ming PEI Fu-xing LIU Lei |
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| Affiliation: | 1. Department of Orthopaedics, the West China Hospital, Sichuan University, Chengdu 610041, China
2. Department of Obstetrics and Gynecology, the West China Hospital, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective: To study the adhesion, migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/ polylactic acid (PLGA) fibres.Methods: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen, polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal microscope.Results: Schwann cell/ECM solution wascompounded with PLA nonspinning fibre cloth. With formation of gel, most Schwann cells resided in the fibre net holes, and adhered to the fibres to form a multiplayer-arranged Schwann cell column like Bungner band. Schwann cells could adhere to PLGA fibres and grew and migrated along the fibres. ECM gel could significantly increase the adhering and migrating cell number.Conclusions: ECM gel can facilitate the adhesion, growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve. |
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| Keywords: | Schwann cells Tissue engineering Three-dimensional culture |
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