脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性

目的：探讨cripto反义寡核苷酸（ASODN）对结肠癌细胞端粒酶活性的影响。 方法: 应用脂质体瞬时转染法介导cripto 反义寡核苷酸，处理人结肠癌细胞系后，分别采用实时定量PCR检测cripto mRNA表达，用TRAP检测端粒酶活性，采用软琼脂集落培养试验检测结肠癌细胞的生长。 结果: Cripto ASODN可有效抑制结肠癌细胞集落生长，且与浓度相关。结肠癌细胞经Cripto ASODN转染后，端粒酶活性明显受到抑制，呈作用浓度和时间依赖性。并与ASODN的浓度和处理时间有关。 结论: cripto基因可能参与对结肠癌细胞端粒酶活性的调控。

Inhibition of telomerase activity in colon cancer LS-174T cells with liposome-mediated cripto antisense oligodeoxynucleotide

AIM: To study the effects of cripto antisense oligodeoxynucleotide (ASODN) on telomerase activity in colon cancer LS-174T cells. METHODS: Colon cancer LS-174T cells were treated with cripto ASODN. The expression of cripto mRNA was studied using real-time PCR. The telomerase activity was detected by telomeric repeat amplification protocol enzyme-linked immunoabsordent assay (TRAP-ELISA). The growth of LS-174T cells was observed with soft agar. RESULTS: Cripto ASODN inhibited the growth of LS-174T cells in a dose-dependent manner in soft agar. Cripto ASODN decreased the telomerase activity in colon cancer LS-174T cells, and the effects of repression were time-and dose-dependent. CONCLUSION: Cripto may contribute to the activation of telomerase in colon cancer.