急性胰腺炎大鼠模型巨嗜细胞移动抑制因子(MIF) 表达变化规律的研究

目的 :本实验旨在观察急性胰腺炎大鼠MIF表达变化规律 ,探讨MIF在急性胰腺炎发病机制中的作用。方法 :使用雄性Wistar大鼠建立急性水肿性胰腺炎 (AEP)与急性坏死性胰腺炎 (ANP)动物模型。分别于Ohr,1hr,2hr,4hr,8hr采集AEP组与ANP组下腔静脉血 ,ANP组腹水标本。ELISA法测定各组标本MIF水平含量。同时检测AEP组与ANP组血清淀粉酶含量。结果 :与正常组相比 ,AEP组血液MIF含量升高 (P <0 0 5 ) ,ANP组血液与腹水MIF含量均升高 (P <0 0 1) ;与正常组相比 ,AEP组与ANP组血清淀粉酶明显升高 ,有统计学意义 (P <0 0 5 )。结论 :急性胰腺炎发病过程中MIF表达明显增加 ,提示在急性胰腺炎发病机制中MIF可能发挥了作用。

The Study of Macrophage Migration Inhibitory Factor(MIF)Expression in Rat Acute Pancreatitis

Objective:The aim of this study was to explore the role of MIF in the pathogenesis of acute pancreatits,observing MIF expression in acute pancreatitis rats.Methods:Acute edematous pancreatitis(AEP)and acute necrotizing pancreatitis(ANP)were induced on male Wistar rats.Blood samples and ascitic fluids in acute pancreatitis rats were collected from the inferior vena cava and the abodominal cavity,respectively,at 0,1,2,4,and 8 hours after the induction of pancreatitis.MIF levels of all samples were determined by enzyme-linked immunosorbent assay(ELISA).Serum amylase levels in blood samples were measured.Results:Compared with those of normal rats,serum MIF levels in AEP rats were increased(P<0.05),and serum and ascitic MIF levels in ANP rats were also increased(P<0.01).Serum amylase levels were higher in AEP and ANP rats than normal rats significantly.Conclusion:MIF expression in acute pancreatitis significantly increased,suggesting a role of MIF in the pathogenesis of acute pancreatitis.