高效液相色谱法测定不同产地不同年份牡丹皮中丹皮酚的含量

目的建立高效液相色谱法,测定不同产地、不同年份牡丹皮中丹皮酚含量。方法色谱柱为Diamonsil C18柱（4.6mm×200.0mm,5.0μm）,流动相为甲醇-醋酸-水（54：2：44）,流速1.0ml/min;检测波长274nm。结果丹皮酚在5.1～102.0μg/ml范围内线性关系良好,r=0.9996,平均回收率为99.4%,精密度（RSD）=0.94%。不同产地及同一产地不同年份牡丹皮中丹皮酚含量差异显著（P〈0.01）。结论该方法准确、稳定、简单,可以作为牡丹皮药材的质量控制方法。

Determination of Paeonol Contents in Cortex Moutan by High Performance Liquid Chromatography (HPLC) from Different Habitats and Different Years

Objective To establish a HPLC method for determination of paeonol in Cortex Moutan from different habitats and different years.Methods The column methods were Diamonsil C18（4.6 mm×200.0 mm,5.0 μm）.The mobile phase was a combination of me-thanol,aceticacid and water in the volume ratio of 54：2：44 at the flow rate of 1 ml/min.The elvation was monitored with UV absorption at 274 nm.The system worked at room temperater.Results The lineal range of Paeonol were 5.1-102.0 μg/ml,r= 0.9996,the mean recovery was 99.4%,and RSD was 0.94%.There was significant difference in contents of paeonol in Cortex Moutan from different habitats and different years（P0.01）.Conclusion This method is accurate,simple and reliable and can be used to control quality of Cortex Moutan.