心肌缺血再灌注损伤中纳洛酮的抗氧化作用

目的 在大鼠离体心脏缺血再灌注模型上,观察纳洛酮对大鼠心肌氧自由基代谢的影响,探讨纳洛酮对心脏作用的可能机制.方法 建立改良的Langendofff离体大鼠心脏灌注模型,缺血40min,再恢复常速灌注30min,造成心肌缺血再灌注损伤.利用该模型观察给予不同浓度的纳洛酮后大鼠心肌SOD活性及MDA含量的变化.结果 模型组与对照组比较,心肌MDA含量增加,SOD活力降低;给予两种不同浓度的纳洛酮分别灌注缺血的心脏后,与对照组比较,纳洛酮16mg/L和32mg/L可减少心肌MDA生成量,并提高SOD活力.结论 纳洛酮对大鼠再灌注损伤心脏的具有保护作用,其机制与抗氧化作用有关.

The Effects of Naloxone in Free Oxygen Radical During Myocardial Ischemia-reperfusion period

Objective TO study the effects of Naloxone in free radial on myocardial ischemia-reperfusion injury.Methords A langendorff rats model were builded,then isolated rat hearts were subjected to ischemia for 40 min and reperfusion for 30 min.myocardial levels f malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)were detected.Results Myocardial enzyme(LDH)and MDA in model group was significantly increase than control group;the activity of SOD was decreased(P<0.01).In the NAL group the levels of MDA and LDH reduced,the activity of SOD significantly higher than model group(P<0.05).Conclusions the results suggested that NAL had protective effects on alleviated reperfusion injury after ischemia,Which might be associated with enhancing the ability of antroxygen free radical.