间充质干细胞条件培养基对糖尿病肾病大鼠肾脏的保护作用

目的利用微量渗透泵持续恒量地向糖尿病肾病(DN)模型大鼠腹腔内泵入间充质干细胞(MSCs)条件培养基,观察其对DN大鼠的保护作用。方法将30只SD大鼠随机分为3组:正常组,DN模型组和MSCs条件培养基组,每组10只。正常组给予普通饲料喂养;DN模型组大鼠每天给予高脂高糖饲料;MSCs条件培养基组是对造模成功的DN大鼠植入Alzet微量渗透泵。检测血糖、血清胰岛素和甘油三酯等指标,观察肾脏病理情况,免疫印迹法(Western blot)检测肾组织nephbin蛋白的表达情况。结果与DN模型组大鼠相比,MSCs条件培养基组的血糖、血清胰岛素、24 h尿蛋白、血肌酐和血尿素氮均显著增高,差异具有统计学意义(P0.05)。MSCs条件培养基组大鼠的肾小球系膜截面积与肾小球体积均显著减小(P0.05)。与正常组大鼠相比,MSCs条件培养基组大鼠nephrin蛋白的表达情况显著降低(P0.05),但与DN模型组相比显著增加(P0.05)。结论微量渗透泵持续泵入MSCs条件培养基可显著改善DN大鼠肾功能。

Renoprotective effect of conditioned medium of marrow mesenchymal stem cells on diabetic nephropathy rats model

Objective To determine the protective effect of conditioned medium from mesenchymal stem cells (MSCs) on the diabetic nephropathy (DN) rats after intraperitoneal osmotic mini-pump implantation. Methods Thirty Sprasue-Dawley (SD) male rats were randomly divided into 3 groups, these are, normal control group, DN model group and MSCs conditioned medium group, with 10 rats in each group. The rats of the normal control group were fed common food, while those in DN group and MSCs group were fed a high-fat high-sucrose diet. The MSCs group was implanted with Alzet osmotic pump after DN models were confirmed. Blood glucose, serum insulin, triglycerides and other indices were tested in the 3 groups of rats. Renal structural damage was evaluated by periodic acid-Schiff (PAS) staining. Expression of nephrin in the renal tissues was detected by Western blotting. Results The blood glucose, serum insulin, 24-hour urinary protein, serum creatinine and blood urea nitrogen were significantly higher in the MSCs group than in the DN group (P<0.05), and the glomerular mesangial area and the glomerular volume were obviously decreased (P<0.05). The MSCs group had significantly decreased expression of nephrin protein than the normal control group (P<0.05), but the level was higher in comparison with that of the DN group (P<0.05). Conclusion Continuous pumping of conditioned medium from mesenchymal stem cells into abdominal cavity exerts a protective effect on the renal function of DN rats.