HPLC法测定哮喘片中盐酸麻黄碱的含量 |
| |
引用本文: | 刘爽,李馨,王金宏,原继红,江蔚新. HPLC法测定哮喘片中盐酸麻黄碱的含量[J]. 黑龙江医药, 2009, 22(5): 595-596 |
| |
作者姓名: | 刘爽 李馨 王金宏 原继红 江蔚新 |
| |
作者单位: | 1. 哈尔滨商业大学 2. 黑龙江大学 |
| |
摘 要: | 目的:应用HPLC法测定哮喘片中盐酸麻黄碱的含量。方法:采用高效液相色谱法;色谱柱为C18柱(200mm×4.6mm,5μm);流动相为乙腈-0.1%磷酸溶液(9:87);流速为1.0ml/min柱温:25℃;检测波长:207砌;进样量:10μl。结果:盐酸麻黄碱在0.04—0.20μg/ml浓度范围内与峰面积线形关系良好,其回归方程为:Y=169373X+22290,r=0.9998。平均回收率为96.96%,RSD为0.12%。结论:本法快速简便,准确。重现性好。
|
关 键 词: | 哮喘片 盐酸麻黄碱 含量测定 |
Determination of Ephedrine Hydrochloride in Asthma Tablets |
| |
Affiliation: | Liu Shung , et al (Harbin University of Commerce Harbin 150076) |
| |
Abstract: | Objective: To establish a HPLC method for the determination of ephedrine hydrochloride in asthma tablets. Methods: HPLC method,the analytical column was C - 18(200 mm ×4. 6 mm,5 μm). The mobile phase consisted of aeetonitrile -0. 1% phosphoric acid(9:87). The flow rate was 1.0 ml/min. The column temperature was at 25℃. The Derector wavelength was set at 207 nm. The injection volume was 10μl. Results:The calibration curve was linear in the range of 0. 04 - 0. 20μg/ml of ephedrine hydroehloride with correlation coefficient of 0. 9998, and the regression equation as Y = 169373X + 22290. The average recovery rate was 96. 96%, and RSD was 0. 55%. Conclusion:The method is quick, accurate, and simple with good repetition. |
| |
Keywords: | Asthma Tablets HPLC content determination Ephedrine hydroehloride |
本文献已被 维普 万方数据 等数据库收录! |
|