p53和nm23-H1在鼻咽癌组织中的表达及其意义

目的 探讨p53和nm23-H1蛋白在鼻咽癌中的表达及其与临床的相关性.方法 应用免疫组织化学方法对鼻咽癌40例、慢性鼻咽炎22例组织中p53和nm23-H1蛋白的表达进行检测,同时与临床相关资料进行对比研究.结果 慢性鼻咽炎组p53及nm23-H1蛋白的阳性率分别为1.0％、27.2％,而鼻咽癌组为92.5％、55.0％.在nm23-H1蛋白表达阳性的22例鼻咽癌组织中p53阳性表达9例（40.9％）,在nm23-H1表达阴性的18例中p53蛋白阳性表达17例（94.4％）.鼻咽癌组织中p53和nm23-H1蛋白的表达明显高于慢性鼻咽炎组织.p53蛋白表达与鼻咽癌淋巴结转移、临床分期和病理学分级呈正相关,与肿瘤T分级无关;nm23-H1蛋白表达与鼻咽癌淋巴结转移及临床分期呈负相关,与肿瘤T分级无关.结论 抑癌基因p53和nm23-H1在鼻咽癌的发生、发展及转移过程中起着协同、调控作用,可成为临床诊断和评价预后的重要生物学标志.

Expression of p53 and nm23-H1 in nasopharyngeal carcinoma and their significance

Objective　To sdudy the expression of p53 and nm23-H1 proteins and their clinical significance in nasopharyngeal carcinoma. Methods　The expression of p53 and nm23-H1 proteins were detected by immunohistochemistry method in 40 cases with nasopharyngeal carcinoma and 22 cases with chronic inflammation of nasopharyngeal mucosa tissues. Results　Positive rates of p53 and nm23-H1 in chronic nasopharyngitis group were 1.0 %, 27.2 %, and in the NPC group were 92.5 %, 55.0 %. There were 9 cases with the positive expression of p53 in 22 cases of nasopharyngeal carcinoma tissues with the positive expression of nm23-H1 (40.9 %). There were 17 cases with the positive expression of p53 in 18 cases with the negative expression of nm23-H1 (94.4 %). The expression of p53 and nm23-H1 proteins in nasopharyngeal carcinoma were much higher than that in chronic inflammation of nasopharyngeal mucosa tissues. The expression of p53 protein in nasopharyngeal carcinoma with metastatic lymph node was higher than that in nasopharyngeal carcinoma without metastatic lymph node, but nm23-H1 protein lower. The expression of p53 protein was positively correlated with the metastasis, clinnical staging and pathological classification but not correlated with T classification of nasopharyngeal carcinoma. The expression of nm23-H1 protein was negative correlation with the metastasis and clinical staging of nasopharyngeal carcinoma. Conclusion　p53 and nm23-H1 play important coordinated regulation roles in the carcinogenesis, development and metastasis of nasopharyngeal carcinoma and will probably become the key biological marks in the judging and evaluating prognosis of nasopharyngeal carcinoma.