射频消融对荷瘤大鼠树突状细胞分化成熟影响因素的探讨

目的 探讨射频消融(RFA)治疗大鼠肝肿瘤前后血清TGF-β1、外周血IL-10的变化及其意义.方法 采用瘤块包埋法制作SD大鼠Walker-256肝肿瘤模型30只,随机分为RFA 1周组、RFA 2周组和对照组,每组10只,前两组分别在RFA后1周、RFA后2周处死动物,对照组不做RFA即处死动物并取外周血,采用Ficoll密度梯度离心法分离外周血单个核细胞,应用ELISA法检测血清TGF-β1,应用FCM检测其IL-10水平.结果 ①对照组大鼠血清TGF-β1水平(6.61±0.12)μg/L.RFA后1周为(5.63±0.46)μg/L,RFA后2周为(5.53±0.56)μg/L,RFA后1周组及RFA后2周组与对照组比较差异均有统计学意义(P＜0.05).②对照组大鼠外周血IL-10表达水平95.92±2.31,RFA后1周为89.71±5.44,RFA后2周为87.67±11.11,RFA后1周组与对照组比较差异有统计学意义(P＜0.05).结论 RFA通过对肿瘤组织的原位灭活在一定程度上解除肿瘤组织所释放的TGF-β1、IL-10对荷瘤宿主的免疫抑制状态,促进荷瘤宿主体内树突状细胞的分化成熟,提高机体抗原提呈能力.

Study of influential factors of radiofrequency ablation on differentiation of dendritic cells of rats with liver tumor

Objective To discuss the change of serum TGF-β1 and IL-10 in peripheral blood of rats with liver tumor treated by RFA.Methods Thirty experimental liver tumor model of SD rats were prepared by implantation of tumor particles.These rats were randomly divided equally into three groups including 1 week after RFA,2 week after RFA and control group.Peripheral blood of control group,group 1 week after RFA and group 2 week after RFA was taken out respectively without RFA,1 week and 2 week after RFA.The mononuclear cells of peripheral blood were separated by Ficoll density gradient centrifugation.The expression level of IL-10 in peripheral blood was analyzed with flowcytometry.Serum TGF-β1 were dectected by ELISA.Results The serum expression level of TGF-β1 of control group was(6.61±0.12)μg/L,and that of group 1 week and 2 week after RFA were respectively(5.63±0.46)μg/L and(5.53±0.56)μg/L.There was statistical significance for the difference between control group and group 1 week or group 2 week after RFA.The serum expression level of IL-10 of control gorup was 95.92±2.31,and that of group 1 week and 2 week after RFA were respectively 89.71±5.44 and 87.67±11.11.There was statistical significance for the difference between control group and group 1 week after RFA.Conclusions RFA can destroy the tumor tissues in situ and relief immune suppression by IL-10,TGF-β1 secreted by tumor tissue.RFA can improve differentiation and maturation of dendritic cell in local area of tumor and promate ability of antigen-presentation of body.